The genome of the diatom Thalassiosira pseudonana:: Ecology, evolution, and metabolism
The genome of the diatom Thalassiosira pseudonana:: Ecology, evolution, and metabolism
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DOI：
10.1126/science.1101156
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发表时间：
2004-10-01
期刊：
影响因子：
56.9
通讯作者：
Rokhsar, DS
中科院分区：
文献类型：
--
作者：
Armbrust, EV;Berges, JA;Rokhsar, DS
硅藻是单细胞藻类，其质体是通过次生内共生获得的。它们对全球碳固定的贡献率接近20%。我们报告了3400万碱基对的海洋硅藻假海藻核基因组草案及其12.9万碱基对的叶绿体和4.4万碱基对的线粒体基因组。序列和光学限制性内切图谱显示24条二倍体核染色体。我们发现了硅藻在水生环境中繁荣生长的新基因，包括硅酸运输和硅基细胞壁形成、高亲和力铁摄取、几种多不饱和脂肪酸的生物合成酶、一系列氮化合物的使用和一个完整的尿素循环。
Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. They are responsible for similar to20% of global carbon fixation. We report the 34 million-base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand-base pair ptastid and 44 thousand-base pair mitochondrial genomes. Sequence and optical restriction mapping revealed 24 diploid nuclear chromosomes. We identified novel genes for silicic acid transport and formation of silica-based cell walls, high-affinity iron uptake, biosynthetic enzymes for several types of polyunsaturated fatty acids, use of a range of nitrogenous compounds, and a complete urea cycle, all attributes that allow diatoms to prosper in aquatic environments.