A Customized Self-Assembling Peptide Hydrogel for Dental Pulp Tissue Engineering
A Customized Self-Assembling Peptide Hydrogel for Dental Pulp Tissue Engineering
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DOI：
10.1089/ten.tea.2011.0222
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发表时间：
2012-01-01
影响因子：
4.1
通讯作者：
D'Souza, Rena N.
中科院分区：
文献类型：
--
作者：
Galler, Kerstin M.;Hartgerink, Jeffrey D.;D'Souza, Rena N.
根管治疗是牙科中的常见做法。在此过程中，发炎或坏死的牙髓被去除并用合成材料代替。然而，最近的研究提供的证据表明，通过使用生物驱动的方法可以对牙髓和牙本质进行工程改造。由于组织工程策略有望很快取代传统的根管治疗，因此需要用于干细胞输送或招募的定制支架。我们假设将牙髓干细胞与生物活性因子掺入这种支架中可以促进细胞增殖、分化和血管生成。在这项研究中，我们使用由自组装肽纳米纤维制成的细胞粘合剂、酶可裂解水凝胶来封装牙髓干细胞。生长因子（GF）、碱性成纤维细胞生长因子、转化生长因子β1和血管内皮生长因子通过肝素结合掺入水凝胶中。建立释放曲线，并评估 GF 对细胞形态和增殖的影响，以确认其结合和随后释放后的生物活性。通过使用细胞追踪器和组织学染色来可视化三维培养物中的细胞形态和扩散。将牙本质柱内的水凝胶皮下移植到免疫功能低下的小鼠体内，导致形成类似于牙髓的血管化软结缔组织。这些数据支持使用这种新型生物材料作为再生牙髓学未来治疗概念的极具前景的候选材料。
Root canal therapy is common practice in dentistry. During this procedure, the inflamed or necrotic dental pulp is removed and replaced with a synthetic material. However, recent research provides evidence that engineering of dental pulp and dentin is possible by using biologically driven approaches. As tissue engineering strategies hold the promise to soon supersede conventional root canal treatment, there is a need for customized scaffolds for stem cell delivery or recruitment. We hypothesize that the incorporation of dental pulp-derived stem cells with bioactive factors into such a scaffold can promote cell proliferation, differentiation, and angiogenesis. In this study, we used a cell adhesive, enzyme-cleavable hydrogel made from self-assembling peptide nanofibers to encapsulate dental pulp stem cells. The growth factors (GFs) fibroblast growth factor basic, transforming growth factor beta 1, and vascular endothelial growth factor were incorporated into the hydrogel via heparin binding. Release profiles were established, and the influence of GFs on cell morphology and proliferation was assessed to confirm their bioactivity after binding and subsequent release. Cell morphology and spreading in three-dimensional cultures were visualized by using cell tracker and histologic stains. Subcutaneous transplantation of the hydrogel within dentin cylinders into immunocompromised mice led to the formation of a vascularized soft connective tissue similar to dental pulp. These data support the use of this novel biomaterial as a highly promising candidate for future treatment concepts in regenerative endodontics.