Heterogeneity of young and aged murine hematopoietic stem cells revealed by quantitative clonal analysis using cellular barcoding
Heterogeneity of young and aged murine hematopoietic stem cells revealed by quantitative clonal analysis using cellular barcoding
复制标题
DOI：
10.1182/blood-2013-01-481135
复制
发表时间：
2013-07-25
期刊：
影响因子：
20.3
通讯作者：
Bystrykh, Leonid V.
中科院分区：
文献类型：
--
作者：
Verovskaya, Evgenia;Broekhuis, Mathilde J. C.;Bystrykh, Leonid V.
造血干细胞（HSC）的数量，有助于血液形成和动态的克隆贡献是一个正在进行的讨论。在这里，我们使用细胞条形码与多重高通量测序相结合，以提供数百个年轻和老年HSC的克隆行为的定量和灵敏度分析。大多数移植的克隆长期稳定地促进造血，尽管粒细胞、T细胞和B细胞中的克隆输出是显著不同的。单个克隆对血液的贡献是动态变化的;大多数克隆随着时间的推移而扩大或减少。最后，我们证明了老年HSC库由多个小克隆组成，而年轻HSC库由较少但较大的克隆占主导地位。
The number of hematopoietic stem cells (HSCs) that contributes to blood formation and the dynamics of their clonal contribution is a matter of ongoing discussion. Here, we use cellular barcoding combined with multiplex high-throughput sequencing to provide a quantitative and sensitive analysis of clonal behavior of hundreds of young and old HSCs. The majority of transplanted clones steadily contributes to hematopoiesis in the long-term, although clonal output in granulocytes, T cells, and B cells is substantially different. Contributions of individual clones to blood are dynamically changing; most of the clones either expand or decline with time. Finally, we demonstrate that the pool of old HSCs is composed of multiple small clones, whereas the young HSC pool is dominated by fewer, but larger, clones.