Label-free, real-time glycosyltransferase assay based on a fluorescent artificial chemosensor.
Label-free, real-time glycosyltransferase assay based on a fluorescent artificial chemosensor.
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DOI：
10.1002/anie.200503107
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发表时间：
2006-01
影响因子：
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通讯作者：
J. Wongkongkatep;Y. Miyahara;A. Ojida;I. Hamachi
中科院分区：
文献类型：
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作者：
J. Wongkongkatep;Y. Miyahara;A. Ojida;I. Hamachi
现实检查：荧光探针1应用于实时糖基转移酶测定。1的荧光随糖基转移反应的进行而增强。该方法在多底物酶促条件下的高灵敏度和可靠的输出使其适合于高通量筛选糖基转移酶抑制剂。（化学方程式）
Reality check: The fluorescent probe 1 is applied to a real-time glycosyltransferase assay. The fluorescence of 1 increases with the progress of the glycosyl transfer reaction. The high sensitivity and reliable output of the method under multisubstrate enzymatic conditions make it suitable for the high-throughput screening of glycosyltransferase inhibitors.(Chemical Equation Presented)