miR-375 is highly expressed and possibly transactivated by achaete-scute complex homolog 1 in small-cell lung cancer cells
miR-375 is highly expressed and possibly transactivated by achaete-scute complex homolog 1 in small-cell lung cancer cells
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miR-375 在小细胞肺癌细胞中高表达，并可能被 achaete-scute 复合物同源物 1 反式激活
DOI：
10.1093/abbs/gmr110
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发表时间：
2012-02-01
影响因子：
3.7
通讯作者：
Zhu, Xueliang
中科院分区：
文献类型：
--
作者：
Zhao, Huijie;Zhu, Lei;Zhu, Xueliang
在本研究中，我们鉴定了五个在小细胞肺癌(SCLC)细胞系NCI-H209中高表达的miRNAs。其中，miR-375在所有SCLC细胞系中的表达水平均显著升高，这与转录因子achaete-scute Complex Homolog 1(ASCL1)的表达一致。此外，miR-375在小鼠SCLC样瘤产生的细胞系中也上调，并与ASCL1相关。双荧光素酶分析进一步表明，ASCL1通过与miR-375启动子中的三个E盒元件结合而激活miR-375的表达。这些结果表明ASCL1通过上调miR-375在小细胞肺癌中发挥作用。
In this study, we identified five miRNAs highly expressed in the small-cell lung cancer (SCLC) cell line NCI-H209. Among them, the expression levels of miR-375 were dramatically elevated in all SCLC cell lines examined, coincident with the expression of the transcription factor achaete-scute complex homolog 1 (ASCL1). Moreover, miR-375 was upregulated and correlated with ASCL1 in the cell lines generated from mouse SCLC-like tumors as well. Dual-luciferase assays further showed that ASCL1 activated the expression of miR-375 by binding to the three E-box elements in the miR-375 promoter. These results imply a role of ASCL1 in SCLC via the upregulation of miR-375.