Regulation of promoter selection by the bacteriophage T7 RNA polymerase in vitro.
Regulation of promoter selection by the bacteriophage T7 RNA polymerase in vitro.
复制标题
体外噬菌体 T7 RNA 聚合酶对启动子选择的调节。
DOI：
10.1093/nar/8.20.4821
复制
发表时间：
1980
影响因子：
14.9
通讯作者：
Carter,AD
中科院分区：
文献类型：
--
作者：
McAllister,WT;Carter,AD
During bacteriophage T7 infection a phage-specified RNA polymerase transcribes the late phage genes in two temporal classes (class II and class III). In this report, we show that the purified phage polymerase discriminates between the class II and class III promotersinvitroas a function of variables that alter the stability of the DNA helix. These variables include ionic strength, temperature, and the presence of denaturing agents such as dimethyl sulfoxide. In general, initiation at the class II promoters is preferentially inhibited as helix stability is increased. Conditions required for the establishment of salt-resistant transcription complexes by the T7 RNA polymerase have been determined; the establishment of stable complexes at the class II promoters requires the synthesis of a longer nascent RNA transcript than does formation of such complexes at the class III promoters. A comparison of the nucleotide sequences of several class II and class III promoters suggests certain features that may be responsible for the different responses of these promoters to helix destabilization. The conservation of structural features that are peculiar to the class II or class III promoters indicates that these features are important in regulation of T7 transcriptioninvivo. Experiments which bear on the physiological significance of these features are discussed.
