NOVEL KAPPA-LIGHT-CHAIN GENE REARRANGEMENTS IN MOUSE LAMBDA-LIGHT CHAIN-PRODUCING LYMPHOCYTES-B
NOVEL KAPPA-LIGHT-CHAIN GENE REARRANGEMENTS IN MOUSE LAMBDA-LIGHT CHAIN-PRODUCING LYMPHOCYTES-B
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DOI：
10.1038/307749a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
SELSING, E
中科院分区：
文献类型：
--
作者：
DURDIK, J;MOORE, MW;SELSING, E
编码 B 淋巴细胞产生的免疫球蛋白的基因由在种系基因组中单独编码并在 B 细胞个体发育过程中通过重组聚集在一起的片段组成。免疫球蛋白轻链有κ和λ两种类型，但在单个B细胞中仅表达一种类型。据认为，在 B 细胞个体发育过程中，κ 基因重组先于 λ 基因重组2,3。我们在此描述了产生两种 λ 的 B 细胞系中发生的不寻常重组，并表明它们参与了成熟 B 细胞中从 κ 到 λ 基因表达的发育转变。这些重组涉及V-J连接但无功能的κ基因的Jκ-Cκ内含子以及在种系中出现在Cκ外显子下游的序列（称为RS，重组序列）。
The genes that encode the immunoglobulin proteins made by B lymphocytes are made up of segments that are separately encoded in the germ-line genome and brought together by recombination during B-cell ontogeny1. There are two types of immunoglobulin light chain,κandλ, but only a single type is expressed in individual B cells. It is thought thatκgene recombination precedesλgene recombination during B-cell ontogeny2,3. We describe here unusual recombinations that have occurred in twoλ-producing B-cell lines and suggest that they are involved in the developmental switch fromκtoλgene expression in maturing B cells. These recombinations involve theJκ–Cκintrons ofV–Jjoined but nonfunctionalκgenes and a sequence that in the germ line occurs downstream of theCκexon (called RS, for recombining sequence).