Disassembly of layer-by-layer films of plasmid DNA and reducible TAT polypeptide
Disassembly of layer-by-layer films of plasmid DNA and reducible TAT polypeptide
复制标题
DOI：
10.1016/j.biomaterials.2006.08.035
复制
发表时间：
2007-01-01
期刊：
影响因子：
14
通讯作者：
Oupicky, David
中科院分区：
文献类型：
--
作者：
Blacklock, Jenifer;Handa, Hitesh;Oupicky, David
本文报道了质粒DNA和一种可还原阳离子多肽的层层（LbL）膜的拆解。为了利用细胞质膜的还原微环境作为一种潜在的触发因素，组装LbL膜使其同时包含DNA和基于TAT的多肽（PTAT），该多肽主链中具有可还原的二硫键。通过接触角测量仪、椭圆偏振仪和原子力显微镜（AFM）对组装和拆解过程进行监测。将PTAT膜的结构与不可还原的聚（L - 赖氨酸）（PLL）膜的结构进行了比较。PTAT膜和PLL膜都呈现指数增长，但接触角在特征值之间交替变化。椭圆偏振仪和原子力显微镜显示，在体外还原环境中，PTAT膜在24小时内逐渐且完全拆解，而PLL膜则不会。这项研究表明了使用可还原LbL膜进行可控DNA传递的潜力。（c）2006爱思唯尔有限公司。保留所有权利。
This paper reports the disassembly of layer-by-layer (LbL) films of plasmid DNA and a reducible cationic polypeptide. To utilize a reducing microenvironment of cellular plasma membrane as a potential trigger, LbL films are assembled to contain both DNA and the TAT-based polypeptide (PTAT) with reducible disulfide bonds in the backbone. The assembly and disassembly processes are monitored by goniometry, ellipsometry, and atomic force microscopy (AFM). The structure of the PTAT films is compared with that of non-reducible poly(L-lysine) (PLL) films. Both PTAT and PLL films exhibit exponential growth but with the contact angle alternating between characteristic values. Ellipsometry and AFM show a gradual and complete disassembly of the PTAT but not the PLL films in a 24 h period in the reducing environment in vitro. This study suggests a potential of using reducible LbL films for controlled DNA delivery. (c) 2006 Elsevier Ltd. All rights reserved.