EXAFS Evidence for a "Cysteine Switch" in the Activation of Prostromelysin
EXAFS Evidence for a "Cysteine Switch" in the Activation of Prostromelysin
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EXAFS 证据证明 Prostromelysin 激活过程中存在“半胱氨酸开关”
DOI：
10.1021/ja00050a048
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发表时间：
1992
影响因子：
15
通讯作者：
L. Que
中科院分区：
文献类型：
--
作者：
R. Holz;S. Salowe;Catherine K. Smith;G. C. Cuca;L. Que
锌K-边缘EXAFS数据的基质金属蛋白酶（MMP）基质溶解素-1的潜在酶原和成熟的活性形式。用2.01 A下每个Zn 4个N/O散射，令人满意地模拟了成熟溶基质素的傅里叶滤波（反变换0.7-2.3 A）χk 3光谱，而由于不合理的DebyeWaller因子或显着较大的拟合残差，前溶基质素的类似拟合被认为是不可接受的
Zn K-edge EXAFS data of the matrix metalloproteinase (MMP) stromelysin-1 were obtained in both its latent proenzyme and mature active forms. The Fourier-filtered (back-transform 0.7-2.3 A) χk 3 spectrum of mature stromelysin was satisfactorily simulated with 4 N/O scatteres per Zn at 2.01 A, while similar fits for prostromelysin were judged unacceptable because of unreasonable DebyeWaller factors or significantly larger residuals of the fits