EXPRESSION OF FAS ANTIGEN ON KERATINOCYTES IN-VIVO AND INDUCTION OF APOPTOSIS IN CULTURED KERATINOCYTES
EXPRESSION OF FAS ANTIGEN ON KERATINOCYTES IN-VIVO AND INDUCTION OF APOPTOSIS IN CULTURED KERATINOCYTES
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DOI：
10.1111/1523-1747.ep12394858
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发表时间：
1994-09-01
影响因子：
6.5
通讯作者：
MIKI, Y
中科院分区：
文献类型：
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作者：
SAYAMA, K;YONEHARA, S;MIKI, Y
Fas抗原属于神经生长因子/肿瘤坏死因子受体超家族，是一种诱导细胞凋亡的膜蛋白。在人类中，已报道Fas抗原分布在细胞系和淋巴细胞上。免疫组化研究显示，Fas抗原在苔藓样药疹、多形性红斑、接触性皮炎、大疱性类天疱疮、寻常天疱疮和带状疱疹的皮损表皮角质形成细胞上表达，它与细胞间粘附分子-1共表达。培养的角质形成细胞经γ-干扰素刺激24 h后，Fas抗原的表达由8.4%增加到34.6%。用抗Fas抗体处理γ-干扰素刺激的角质形成细胞48 h，导致32%的细胞DNA断裂和死亡，表明Fas抗原可能介导细胞凋亡。皮损角质形成细胞表面Fas抗原的表达提示Fas抗原介导的细胞死亡在角质形成细胞毒性的发病机制中起重要作用。
Fas antigen, which belongs to a nerve growth factor/tumor necrosis factor receptor superfamily, is a membrane protein that induces apoptosis. In humans, distribution of Fas antigen has been reported on cell lines and lymphocytes. Immunohistochemical studies revealed Fas antigen on the keratinocytes of lesional epidermis in lichenoid drug eruption, erythema multiforme, contact dermatitis, bullous pemphigoid, pemphigus vulgaris, and herpes zoster; it is co-expressed with intercellular adhesion molecule-1. Cultured keratinocytes expressing Fas antigen increased from 8.4% to 34.6% after stimulation with interferon gamma for 24 h. Treatment of interferon-gamma-stimulated keratinocytes with anti-Fas for 48 h resulted in DNA fragmentation and death of 32% of cells, suggesting that Fas antigen may mediate apoptosis. The expression of Fas antigen on keratinocytes in lesional skin suggests that death via Fas antigen may play an important role in the pathogenesis of keratinocyte cytotoxicity.