Manipulation and sorting of membrane proteins using patterned diffusion-aided ratchets with AC fields in supported lipid bilayers
Manipulation and sorting of membrane proteins using patterned diffusion-aided ratchets with AC fields in supported lipid bilayers
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DOI：
10.1039/c2sm25473e
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发表时间：
2012-01-01
期刊：
影响因子：
3.4
通讯作者：
Evans, Stephen D.
中科院分区：
文献类型：
--
作者：
Cheetham, Matthew R.;Bramble, Jonathan P.;Evans, Stephen D.
我们提出的棘轮能够指导支持双层脂质膜内的带电成分的运动。这些棘轮利用不对称图案化特征和AC电场，并且已经被证明在两个储库之间运输带电物质，例如脂质和跨膜蛋白。蛋白质在膜中以两种方向存在，只有那些膜外结构域远离玻璃基底的蛋白质是移动的。使用这些棘轮运输移动的方向的蛋白质，从而将两个方向彼此分选，并产生一个方向上的蛋白质比另一个方向上的蛋白质多五倍的膜区域。
We present ratchets capable of directing the movement of charged components within supported bilayer lipid membranes. These ratchets make use of asymmetrically patterned features and AC electric fields, and have been demonstrated to transport charged species such as lipids and transmembrane proteins between two reservoirs. Proteins were present in both orientations in the membrane, with only those with their extra-membranous domain orientated away from the glass substrate being mobile. Proteins in the mobile orientation were transported using these ratchets, thereby sorting the two orientations from one another, and creating an area of the membrane containing five times more protein in one orientation than the other.