Genome-wide DNA methylation analysis revealed stable DNA methylation status during decidualization in human endometrial stromal cells
Genome-wide DNA methylation analysis revealed stable DNA methylation status during decidualization in human endometrial stromal cells
复制标题
全基因组DNA甲基化分析显示人子宫内膜基质细胞在蜕膜化过程中DNA甲基化状态稳定。
DOI：
10.1186/s12864-019-5695-0
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发表时间：
2019-04-29
期刊：
影响因子：
4.4
通讯作者：
Sugino, Norihiro
中科院分区：
文献类型：
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作者：
Maekawa, Ryo;Tamura, Isao;Sugino, Norihiro
背景在子宫内膜基质细胞（ESC）的蜕膜化过程中，许多基因的表达和组蛋白的表观遗传修饰发生改变。然而，关于DNA甲基化（另一种表观遗传机制）在蜕膜化过程中是否也会发生变化的信息很少。在这里，我们检查了ESC在蜕膜化过程中的全基因组DNA甲基化谱及其与基因表达和组蛋白修饰变化的关系。结果将ESC与雌二醇和醋酸甲羟孕酮一起孵育14天以诱导蜕膜化。比较了非蜕膜化 ESC 和蜕膜化 ESC 之间的全基因组 DNA 甲基化谱。在 482,005 个 CpG 中，只有 23 个 CpG (0.0048%) 显示出不同的 DNA 甲基化状态。还比较了 ESC 之间差异表达基因和具有不同组蛋白修饰（H3K4 三甲基化和 H3K27 乙酰化）的区域的 DNA 甲基化状态。在蜕膜化ESC中上调和下调的基因中，基因启动子区域周围的DNA甲基化状态在ESC之间没有显着差异。在具有不同组蛋白修饰的区域中，ESC 之间的 DNA 甲基化状态没有差异。差异表达基因和差异组蛋白修饰区域低甲基化。结论用雌激素/孕激素培养ESCs并没有扭曲DNA甲基化的生理模式，尽管mRNA表达和组蛋白修饰发生了动态改变。全基因组 DNA 甲基化分析揭示了人类子宫内膜基质细胞蜕膜化过程中稳定的 DNA 甲基化状态。 DNA 低甲基化因蜕膜化过程中组蛋白修饰和基因表达的可变变化而得以维持。
BackgroundDuring decidualization in endometrial stromal cells (ESCs), expressions of a number of genes and epigenetic modifications of histones are altered. However, there is little information about whether DNA methylation, which is another epigenetic mechanism, also changes during decidualization. Here, we examined the genome-wide DNA methylation profiles in ESCs during decidualization and their associations with the changes of gene expressions and histone modifications.ResultsESCs were incubated with estradiol and medroxyprogesterone acetate for 14days to induce decidualization. The genome-wide DNA methylation profiles were compared between the non-decidualized ESCs and the decidualized ESCs. Of 482,005 CpGs, only 23 CpGs (0.0048%) showed different DNA methylation statuses. The DNA methylation statuses of the differentially expressed genes and the regions with different histone modifications (H3K4 tri-methylation and H3K27 acetylation) were also compared between the ESCs. In the upregulated and downregulated genes in decidualized ESCs, DNA methylation statuses around the promoter region of the genes did not significantly differ between the ESCs. In the regions with different histone modification, DNA methylation statuses did not differ between the ESCs. The differentially expressed genes and the differential histone modification regions were hypomethylated.ConclusionsCulturing ESCs with estrogen/progesterone did not distort the physiological pattern of DNA methylation, although mRNA expression and histone modifications were dynamically altered. A genome-wide DNA methylation analysis revealed stable DNA methylation statuses during decidualization in human endometrial stromal cells. DNA hypomethylation is maintained for the variable changes of histone modifications and gene expression during decidualization.