Apoptosis induction by aluminum phthalocyanine tetrasulfonate-based sonodynamic therapy in HL-60 cells
Apoptosis induction by aluminum phthalocyanine tetrasulfonate-based sonodynamic therapy in HL-60 cells
复制标题
基于四磺酸铝酞菁的声动力疗法诱导 HL-60 细胞凋亡
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
S. Umemura
中科院分区：
文献类型：
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作者：
Y. Iwase;N. Yumita;K. Nishi;Hiroyuki Kuwahara;T. Fukai;T. Ikeda;Fu;Y. Momose;S. Umemura
本研究的目的是研究使用酞菁、酞菁四磺酸氯铝（AlPcTS）的声动力学诱导的细胞凋亡。HL-60细胞暴露于超声长达3分钟的存在和不存在的AlPcTS。通过细胞形态学、DNA片段化和caspase-3活性分析细胞凋亡。电子自旋共振用于测量活性氧。联合治疗（超声和AlPcTS）后显示膜起泡和细胞收缩的凋亡细胞数量显著高于其他治疗（包括单独超声和单独AlPcTS）。此外，在经超声和AlPcTS处理的细胞中观察到DNA梯状条带形成、caspase-3活化和增强的一氧化氮产生。声动力学诱导的细胞凋亡，caspase-3的激活，和氮氧化物的产生显着抑制组氨酸。组氨酸的显着减少表明，超声波产生的活性氧，如单线态氧，是声动力学诱导的细胞凋亡的重要介质。
The present study aims to investigate sonodynamically-induced apoptosis using the phthalocyanine, chloroaluminum phthalocyanine tetrasulfonate (AlPcTS). HL-60 cells were exposed to ultrasound for up to 3 min in the absence and presence of AlPcTS. Apoptosis was analyzed by cell morphology, DNA fragmentation, and caspase-3 activity. Electron spin resonance was used to measure reactive oxygen species. The number of apoptotic cells showing membrane blebbing and cell shrinkage after combined treatment (ultrasound and AlPcTS) was significantly higher than following other treatments, including ultrasound alone and AlPcTS alone. Furthermore, DNA ladder formation, caspase-3 activation and enhanced nitroxide generation were observed in cells treated with ultrasound and AlPcTS. Sonodynamically induced apoptosis, caspase-3 activation, and nitroxide generation were significantly suppressed by histidine. The significant reduction by histidine indicated that ultrasonically generated reactive oxygen species, such as singlet oxygen, is an important mediator of sonodynamically-induced apoptosis.