Application of INAA to the build-up and clearance of gold nanoshells in clinical studies in mice
Application of INAA to the build-up and clearance of gold nanoshells in clinical studies in mice
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DOI：
10.1007/s10967-007-0230-1
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发表时间：
2007-02-01
影响因子：
1.6
通讯作者：
Payne, J. D.
中科院分区：
文献类型：
--
作者：
James, W. D.;Hirsch, L. R.;Payne, J. D.
已经进行了临床研究，以详细测量工程金纳米壳在给药小鼠组织中的积聚和清除。这些光学可调纳米壳正在考虑用于肿瘤的新疗法。所提出的疗法将涉及纳米壳的注射及其在肿瘤部位的优先积累。随后将用单色近红外激光照射，这将诱导细胞热疗，从而根除肿瘤。中子活化分析已用于在给药后的不同处死时间检测和定量给药材料、血液、骨骼和其他组织以及肿瘤中的金，并因此检测和定量纳米壳。可行性研究表明，仪器中子活化分析是唯一适合于检测的金纳米壳在一个很宽的动态范围。这使得研究高浓度的金在组织中，从血液（肝，脾，肾），以及低得多的浓度在那些不（肌肉，脑）的壳。特别是，在最长给药后延迟（28天）后处死的动物和对照动物的组织需要进行实验优化，以确保尽可能低的测定限。组织样品中的金质量范围从我们的测定限（约70 pg）到几微克。
Clinical studies have been carried out for detailed measurements of the build-up and clearance of engineered gold nanoshell in the tissues of dosed mice. These optically tunable nanoshells are under consideration for a new therapy for tumors. The proposed therapy would involve the injection of the nanoshells and their preferential accumulation in tumor sites. This will be followed by irradiation with a monochromatic near infrared laser, which will induce cellular hyperthermia, thereby eradicating the tumor. Neutron activation analysis has been used for the detection and quantitation of gold, and therefore, the nanoshells, in dosing materials, blood, bones and other tissues as well as tumors at varioius sacrifice times following dosing. Feasibility studies have shown instrumental neutron activation analysis to be uniquely suited for detection of the gold nanoshells over a wide dynamic range. This allows for the study of high concentrations of gold in tissues which scavenge the shells from the blood (liver, spleen, kidney) as well as for much lower concentrations in those which do not (muscle, brain). In particular, the tissues from animals sacrificed after the longest post dose delay (28 days) and the control animals required experimental optimization to ensure the lowest possible determination limits. The mass of gold in the tissue samples ranged from our determination limit (about 70 pg) to a few micrograms.