Direct electron microscopy studies of the bone-hydroxylapatite interface.
Direct electron microscopy studies of the bone-hydroxylapatite interface.
复制标题
骨-羟基磷灰石界面的直接电子显微镜研究。
DOI：
10.1002/jbm.820180702
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Doremus,RH
中科院分区：
文献类型：
--
作者：
Tracy,BM;Doremus,RH
在透射电子显微镜下直接观察了骨-羟基磷灰石界面。采用多种电子显微镜技术对骨-羟基磷灰石界面进行了表征，包括明场和暗场成像、电子衍射、高分辨率成像和扫描-透射电子显微镜(STEM-EDS)的能量色散分析。机械研磨后的氩离子研磨产生了对电子透明的未染色和未脱钙的大鼠骨和羟基磷灰石的界面区域。因此，可以确定界面的确切位置，并研究界面处的骨骼。常规和高分辨率的扫描电子显微镜显示骨和羟基磷灰石之间有直接的化学结合。陶瓷表面的骨与远离界面的正常骨基本一致。
The bone‐hydroxylapatite interface has been examined directly in the transmission electron microscope (TEM). The bone—hydroxylapatite interface was characterized by using several electron microscopy techniques, including bright and dark field imaging, electron diffraction, high‐resolution imaging, and energy dispersive analysis in the scanning‐transmission electron microscope (STEM EDS). Mechanical grinding followed by argon‐ion milling produced interface regions of unstained and undecalcified rat bone and hydroxylapatite that were transparent to electrons. Thus the exact location of the interface could be established and the bone at the interface studied. Conventional and high‐resolution imaging in the TEM demonstrated direct chemical bonding between bone and hydroxylapatite. The bone at the ceramic surface was the same as normal bone away from the interface.