Comparative genomic hybridization analysis reveals 3q gain resulting in genetic alteration in 3q in advanced oral squamous cell carcinoma
Comparative genomic hybridization analysis reveals 3q gain resulting in genetic alteration in 3q in advanced oral squamous cell carcinoma
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DOI：
10.1016/s0165-4608(00)00430-1
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发表时间：
2001-05-01
影响因子：
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通讯作者：
Sasaki, K
中科院分区：
文献类型：
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作者：
Oga, A;Kong, G;Sasaki, K
我们用比较基因组杂交技术分析了17例原发性口腔鳞状细胞癌（OSCC）的DNA序列拷贝数畸变（DSCNAs）。3q 25-qter（7/17）、Xp 21（5/17）、Xq 12-q23和8 q23-q24（4/17）是DSCNAs的高发区。13 q21-q22（5/17）、3 p21-pter、4p 15-pter和17 p13（4/17）、Sp22-pter和9 p21-pter（3/17）缺失频率较高。4例肿瘤有7个位点扩增：3q 11-qter。3q13，3q26。7q21-q22、8q23-qter、9p22-pter。12p11 DSCNAs的总数在III期和IV期肿瘤中显著高于I期和II期肿瘤（P= 0.008）。此外，在III期和IV期肿瘤（6/8）中优先检测到3q增益，而不是在I期和II期肿瘤（1/9，P=.013）。在我们的研究中，所有获得3q的肿瘤在共同区域也包含一个或多个丢失。另一方面，所有具有9 p增益的肿瘤不包含3q增益。这些观察结果表明，获得的3q和积累的DSCNAs与口腔鳞状细胞癌的肿瘤进展密切相关。此外，3q常见畸变区域中一个或多个额外位点的增加和丢失似乎是一组与导致晚期OSCC的显性遗传途径相关的DSCN。(C)2001 Elsevier Science，Inc，保留所有权利。
We analyzed DNA sequence copy number aberrations (DSCNAs) in 17 primary oral squamous cell carcinomas (OSCCs) by comparative genomic hybridization. DSCNAs were detected frequently at 3q25-qter (7/17), Xp21 (5/17), and Xq12-q23 and 8q23-q24 (4/17). and losses were detected frequently at 13q21-q22 (5/17), 3p21-pter, 4p15-pter and 17p13 (4/17), and Sp22-pter and 9p21-pter (3/17). Four tumors showed amplifications of seven loci: 3q11-qter. 3q13, 3q26. 7q21-q22, 8q23-qter, 9p22-pter. and 12p11. The total number of DSCNAs was significantly greater in stage III and stage IV tumors than in stage I and stage II tumors (P=.008). Furthermore, 3q gain was detected preferentially in stage III and stage IV tumors (6/8) rather than in stage I and stage II tumors (1/9, P=.013). In our study, all tumors with gain of 3q also contained one or more loss(es) in common regions. On the other hand, all tumors with gain of 9p did not contain 3q gains. These observations indicate that gain of 3q and accumulation of DSCNAs are strongly associated with tumor progression in OSCC. Furthermore, 3q gain and loss of one or more additional loci in common aberration regions appears to be a group of DSCNs associated with dominant genetic pathways of leading to advanced OSCCs. (C) 2001 Elsevier Science, Inc, All rights reserved.