AUTOMATED DETERMINATION OF SERUM IRON.
AUTOMATED DETERMINATION OF SERUM IRON.
复制标题
自动测定血清铁。
作者：
B. Zak;E. Epstein
本文介绍了一种血清铁浓度的自动测定方法。将在抗坏血酸存在下通过酸处理从血清转铁蛋白释放的铁透析到缓冲液中，所述缓冲液能够用4，7-二苯基-1，10-菲咯啉磺酸盐将扩散液维持在适于瞬时和完全颜色形成的pH。程序的细节，铁的扩散性，精度和准确性进行了描述。
An automated procedure for measurement of serum iron concentration is presented. Iron released from the serum transferrin by acid treatment in the presence of ascorbic acid was dialyzed into a buffer capable of maintaining the diffusate at a pH suitable for instantaneous and complete color formation with 4,7-diphenyl-1,10 phenanthroline sulfonate. Procedural details, diffusability of iron, precision, and accuracy are described.