Identification of full-length proviral DNA of porcine endogenous retrovirus from Chinese Wuzhishan miniature pigs inbred
Identification of full-length proviral DNA of porcine endogenous retrovirus from Chinese Wuzhishan miniature pigs inbred
复制标题
中国五指山小型猪近交系猪内源性逆转录病毒全长前病毒DNA的鉴定。
DOI：
10.1016/j.cimid.2008.10.007
复制
发表时间：
2010-07-01
影响因子：
2
通讯作者：
Zhang, Jingang
中科院分区：
文献类型：
--
作者：
Ma, Yuyuan;Lv, Maomin;Zhang, Jingang
猪内源性逆转录病毒（PERV）的存在阻碍了猪在临床异种移植中的应用，以缓解人体移植的短缺。中国小型猪是异种器官移植的潜在供体。然而，到目前为止，关于中国小型猪PERV的分子特征还没有足够的信息。本文报道了五指山小型猪近交系PERV原病毒全长DNA的克隆与鉴定。对PERV-WZSP和其他perv的全长核苷酸序列进行比对，并根据推导出的env氨基酸序列构建了系统发育树。结果表明，PERV-WZSP的全长原病毒DNA属于γ突变体，与其他perv具有较高的相似性。序列分析还表明，在同一种猪种系中存在不同的LTR模式，部分PERV-C序列可能与LTR中的PERV-A序列重组。版权所有。
Existence of porcine endogenous retrovirus (PERV) hinders pigs to be used in clinical xeno-transplantation to alleviate the shortage of human transplants. Chinese miniature pigs are potential organ donors for xenotransplantation in China. However, so far, an adequate level of information on the molecular characteristics of PERV from Chinese miniature pigs has not been available. We described here the cloning and characterization of full-length proviral DNA of PERV from Chinese Wuzhishan miniature pigs inbred (WZSP). Full-length nucleotide sequences of PERV-WZSP and other PERVs were aligned and phylogenetic tree was constructed from deduced amino-acid sequences of env. The results demonstrated that the full-length proviral DNA of PERV-WZSP belongs to gammaretrovints and shares high similarity with other PERVs. Sequence analysis also suggested that different patterns of LTR existed in the same porcine germ line and partial PERV-C sequence may recombine with PERV-A sequence in LTR. (C) 2008 Elsevier Ltd. All rights reserved.