UP-REGULATION OF HEPATOCYTE GROWTH-FACTOR GENE-EXPRESSION BY INTERLEUKIN-1 IN HUMAN SKIN FIBROBLASTS
UP-REGULATION OF HEPATOCYTE GROWTH-FACTOR GENE-EXPRESSION BY INTERLEUKIN-1 IN HUMAN SKIN FIBROBLASTS
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DOI：
10.1016/0006-291x(92)92375-8
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发表时间：
1992-10-15
影响因子：
3.1
通讯作者：
NAKAMURA, T
中科院分区：
文献类型：
--
作者：
MATSUMOTO, K;OKAZAKI, H;NAKAMURA, T
肝细胞生长因子（HGF）作为肝和肾再生因子发挥作用，促进肝脏和肾脏的再生。当将 1 ng/ml 白介素-1α (IL-1α) 或白细胞介素-1β (IL-1β) 添加到人皮肤成纤维细胞培养物中时，HGF 的产生量比对照水平高 5-6 倍。暴露于 IL-1α 后 6 小时，细胞中的 HGF mRNA 水平增加至 4 倍。肿瘤坏死因子-α 和干扰素-γ 对 HGF 产生有轻微的刺激作用，但没有测试其他细胞因子。肿瘤促进剂十四烷酰佛波醇 13-乙酸酯 (TPA) 显着增强了 IL-1α 和 IL-1β 对 HGF 产生的刺激作用。 10 ng/ml TGF-β 1 或 1 μM 地塞米松完全消除了 IL-1α 和 IL-1β 的刺激作用以及与 TPA 的协同刺激作用。这些结果表明，IL-1α和IL-1β是HGF基因表达的正调节因子，并且可能在炎症性疾病发生后的组织再生中发挥作用。
Hepatocyte growth factor (HGF) functions as a hepatotrophic and renotrophic factor for regeneration of the liver and kidney. When 1 ng/ml of interleukin-1α (IL-1α) or interleukin-1β (IL-1β) was added to cultures of human skin fibroblasts, the production of HGF was 5–6 fold higher than levels in the controls. HGF mRNA level in the cells was increased to 4-fold higher levels at 6 h after exposure to IL- 1α. Tumor necrosis factor-α and interferon-γ but no other cytokine tested had slightly stimulatory effects on HGF production. The tumor promoter, tetradecanoylphorbol 13-acetate (TPA) markedly enhanced the stimulatory effect of IL-1α and IL-1β on the production of HGF. The stimulatory effect of both IL-1α and IL-1β and the synergistical stimulation with TPA were completely abrogated by 10 ng/ml TGF-β 1 or 1 μM dexamethasone. These results suggest that IL-1α and IL-1β are positive regulators for expression of the HGF gene and are likely have a role in regeneration of tissues following the occurrence of inflammatory diseases.