Extracellular fatty acids facilitate flagella-independent translocation by Stenotrophomonas maltophilia
Extracellular fatty acids facilitate flagella-independent translocation by Stenotrophomonas maltophilia
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DOI：
10.1016/j.resmic.2007.09.002
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发表时间：
2007-10-01
影响因子：
2.6
通讯作者：
Wong, Amy C. Lee
中科院分区：
文献类型：
--
作者：
Huang, Tzu-Pi;Wong, Amy C. Lee
嗜麦芽窄食单胞菌广泛存在于土壤、污水和植物根际等自然环境中。表面活性剂通常在调节细菌表面移位中起作用。在这项研究中，rpfB和rpfF直向同源物从S.嗜麦芽窄食单胞菌菌株WR-C，其分离自化粪池系统的堵塞区。这些基因在8种胞外化合物的生物合成中发挥作用，这些化合物促进了野生型或鞭毛缺陷型突变体的鞭毛独立易位。这种类型的表面易位以前没有报道过这种生物体。这八个化合物包括顺式-Δ 2-11-甲基-十二碳烯酸和七个结构衍生物。其中两种是饱和脂肪酸;其他的是在2位有双键的不饱和脂肪酸。这些脂肪酸的链长从12个碳到14个碳不等，支链甲基的位置也不同。结果表明，cis-Delta 2-11-甲基-十二碳烯酸和11-甲基-十二烷酸分别促进了S.嗜麦芽窄食单胞菌菌株WR-C作为润湿剂。(C)2007年，Elsevier Masson SAS。All rights reserved.
Stenotrophomonas maltophilia is widespread in natural environments such as soil, sewage and plant rhizospheres. Surfactants frequently function in modulating bacterial surface translocation. In this study, rpfB and rpfF orthologues were identified from S. maltophilia strain WR-C, which was isolated from the clogged zone of a septic system. These genes play a role in the biosynthesis of eight extracellular compounds that facilitated flagella-independent translocation by the wild-type or a flagella-defective mutant. This type of surface translocation has not been reported previously for this organism. These eight compounds include cis-Delta 2-11-methyl-dodecenoic acid and seven structural derivatives. Two are saturated fatty acids; the others are unsaturated fatty acids with double bonds at position 2. These fatty acids vary in chain length from 12 to 14 carbons and in the position of the branched methyl group. Our results demonstrated that independently cis-Delta 2-11-methyl-dodecenoic acid and 11-methyl-dodecanoic acid promoted flagella-independent translocation by S. maltophilia strain WR-C by acting as wetting agents. (C) 2007 Elsevier Masson SAS. All rights reserved.