Insect transferrin functions as an antioxidant protein in a beetle larva
Insect transferrin functions as an antioxidant protein in a beetle larva
复制标题
DOI：
10.1016/j.cbpb.2008.02.009
复制
发表时间：
2008-06-01
影响因子：
2.2
通讯作者：
Jin, Byung Rae
中科院分区：
文献类型：
--
作者：
Kim, Bo Yeon;Lee, Kwang Sik;Jin, Byung Rae
在昆虫中，转铁蛋白被称为铁转运蛋白、抗生素剂、卵黄蛋白原和保幼激素调节蛋白。在这里，一个新的功能作用，昆虫转铁蛋白作为一种抗氧化蛋白被证明。应激，如热休克，真菌的挑战，和H2O2暴露，导致上调的白点花金龟子Protaetia brevitarsis（鞘翅目：金龟子科）转铁蛋白（PbTf）mRNA的脂肪体和增加PbTf蛋白水平的血淋巴。RNA干扰（RNAi）处理的PbTf减少导致血淋巴中铁和H2O2水平增加，并导致在暴露于应激期间诱导脂肪体中的凋亡细胞死亡。观察到的PbTf RNAi的效果表明，PbTf抑制应激诱导的细胞凋亡，通过减少芬顿反应通过铁的结合，从而支持PbTf在应激反应中的抗氧化作用。(c)2008年爱思唯尔公司All rights reserved.
In insects transferrin is known as an iron transporter, an antibiotic agent, a vitellogenin, and a juvenile hormone regulated protein. Here, a novel functional role for insect transferrin as an antioxidant protein is demonstrated. Stressors, such as heat shock, fungal challenge, and H2O2 exposure, cause upregulation of the white-spotted flower chafer Protaetia brevitarsis (Coleoptera: Scarabaeidae) transferrin (PbTf) mRNA in the fat body and increases PbTf protein levels in the hemolymph. RNA interference (RNAi) treated PbTf reduction causes increased iron and H2O2 levels in the hemolymph and results in induction of apoptotic cell death in the fat body during exposure to stress. The observed effects of PbTf RNAi suggest that PbTf inhibits stress-induced apoptosis by diminishing the Fenton reaction via the binding of iron, thus supporting an antioxidant role for PbTf in stress responses. (c) 2008 Elsevier Inc. All rights reserved.