Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway
Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway
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DOI：
10.2478/s11756-009-0040-8
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发表时间：
2009-04-01
期刊：
影响因子：
1.5
通讯作者：
Tang, Kexuan
中科院分区：
文献类型：
--
作者：
Jing, Fuyuan;Zhang, Ling;Tang, Kexuan
青蒿素是从黄花蒿中提取的一种倍半萜内酯内酯，是目前最有效的抗疟疾药物。为了提高青蒿素的产量，用不同浓度的脱落酸(ABA)(1、10和100A/M)处理黄花蒿。结果表明，ABA处理植株的青蒿素含量显著增加。尤其是10A亩M-ABA处理植株的青蒿素含量比对照高65%，平均干重达1.84%。基因表达分析表明，在ABA处理的植株和悬浮培养细胞中，青蒿素生物合成途径中的重要基因HMGR、FPS、CYP71AV1和CPR均显著诱导。在ABA处理的植株中只观察到ADS的表达略有增加，而在悬浮细胞培养中没有检测到ADS的表达。本研究表明，ABA信号通路与青蒿素生物合成途径之间可能存在串扰，其中诱导作用最强的细胞色素P71AV1可能在青蒿素生物合成途径中起着关键的调控作用。
Artemisinin, a sesquiterpene lactone endoperoxide derived from Artemisia annua L., is the most effective antimalarial drug. In an effort to increase the artemisinin production, abscisic acid (ABA) with different concentrations (1, 10 and 100 A mu M) was tested by treating A. annua plants. As a result, the artemisinin content in ABA-treated plants was significantly increased. Especially, artemisinin content in plants treated by 10 A mu M ABA was 65% higher than that in the control plants, up to an average of 1.84% dry weight. Gene expression analysis showed that in both the ABA-treated plants and cell suspension cultures, HMGR, FPS, CYP71AV1 and CPR, the important genes in the artemisinin biosynthetic pathway, were significantly induced. While only a slight increase of ADS expression was observed in ABA-treated plants, no expression of ADS was detected in cell suspension cultures. This study suggests that there is probably a crosstalk between the ABA signaling pathway and artemisinin biosynthetic pathway and that CYP71AV1, which was induced most significantly, may play a key regulatory role in the artemisinin biosynthetic pathway.