Challenges and perspectives in the application of isothermal DNA amplification methods for food and water analysis
Challenges and perspectives in the application of isothermal DNA amplification methods for food and water analysis
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DOI：
10.1007/s00216-018-1553-1
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发表时间：
2019-03-01
影响因子：
4.3
通讯作者：
Reischer, Georg H.
中科院分区：
文献类型：
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作者：
Martzy, Roland;Kolm, Claudia;Reischer, Georg H.
分子诊断工具在食品和水质分析领域正变得越来越普遍。通常，基于聚合酶链式反应(PCR)等DNA扩增技术，这些方法高度敏感，用途广泛，但需要装备精良的实验室和训练有素的人员。为了缩短分析时间，避免昂贵的设备，人们开发了用于检测各种目标生物的等温DNA扩增方法。然而，为了使分子诊断适用于低资源环境和现场应用，关键是不断调整与DNA扩增相关的工作步骤，即样品准备、DNA提取和结果可视化。近年来，人们评估了许多应对这些挑战的新方法，例如，使用离子液体从与食品和水分析有关的生物体中快速分离核酸，或将整个分析工作流程整合到微流控芯片上。无论如何，未来在等温放大领域的应用很可能在于与负担得起的手持设备相结合的即用盒，用于现场分析。这篇趋势文章旨在让潜在用户更熟悉这项技术及其将分子诊断从实验室转移到现场的潜力。
Molecular diagnostic tools in the field of food and water quality analysis are becoming increasingly widespread. Usually, based on DNA amplification techniques such as polymerase chain reaction (PCR), these methods are highly sensitive and versatile but require well-equipped laboratories and trained personnel. To reduce analysis time and avoid expensive equipment, isothermal DNA amplification methods for detecting various target organisms have been developed. However, to make molecular diagnostics suitable for low-resource settings and in-field applications, it is crucial to continuously adapt the working steps associated with DNA amplification, namely sample preparation, DNA extraction, and visualization of the results. Many novel approaches have been evaluated in recent years to tackle these challenges, e.g., the use of ionic liquids for the rapid isolation of nucleic acids from organisms relevant for food and water analysis or the integration of entire analytical workflows on microfluidic chips. In any event, the future of applications in the field of isothermal amplification will probably lie in ready-to-use cartridges combined with affordable handheld devices for on-site analysis. This trend article aims to make prospective users more familiar with this technology and its potential for moving molecular diagnostics from the laboratory to the field.