The Role of PinX1 in Growth Control of Breast Cancer Cells and Its Potential Molecular Mechanism by mRNA and lncRNA Expression Profiles Screening
The Role of PinX1 in Growth Control of Breast Cancer Cells and Its Potential Molecular Mechanism by mRNA and lncRNA Expression Profiles Screening
复制标题
通过mRNA和lncRNA表达谱筛选PinX1在乳腺癌细胞生长控制中的作用及其潜在分子机制
DOI：
10.1155/2014/978984
复制
发表时间：
2014-01-01
影响因子：
--
通讯作者：
Ma, Wen-Li
中科院分区：
文献类型：
--
作者：
Shi, Rong;Zhou, Jue-Yu;Ma, Wen-Li
作为主要抑癌基因，PinX1在乳腺癌中的作用及其分子机制尚不清楚。在这项研究中，PinX1 在 3 种乳腺癌细胞系中过度表达，并在非致瘤性乳腺癌细胞系中敲低 PinX1。观察PinX1对细胞增殖和细胞周期的调节作用。还进行了基于微阵列的 lncRNA 和 mRNA 表达谱筛选。我们发现 PinX1 过表达的乳腺癌细胞的生长速率较低、G0/G1 期停滞和 S 期抑制，而 PinX1 敲低的非致瘤性乳腺癌细胞的生长速率较高、G0/G1 期减少和 S 期速率增加。总共 977 个 mRNA 和 631 个 lncRNA 被鉴定为 PinX1 过表达和对照 MCF-7 细胞之间差异表达的转录本。进一步的分析确定了这些 mRNA 参与 52 条癌症相关途径和各种其他生物过程。 11 个增强子样 lncRNA 和 25 个 lincRNA 及其相邻 mRNA 对被鉴定为共调控转录本。我们的研究结果证实了PinX1作为乳腺癌细胞系中主要抑癌基因的作用，为进一步研究PinX1在肿瘤发生中的分子机制提供了信息。
As a major tumor suppressor gene, the role of PinX1 in breast cancer and its molecular mechanism remain unclear. In this study, overexpression of PinX1 was generated in 3 breast cancer cell lines, and knockdown of PinX1 was performed in a nontumorigenic breast cell line. The regulation of PinX1 on cell proliferation and cell cycle was observed. A microarray-based lncRNA and mRNA expression profile screening was also performed. We found a lower growth rate, G0/G1 phase arrest, and S phase inhibition in the PinX1 overexpressed breast cancer cells, while a higher growth rate, decreased G0/G1 phase, and increased S phase rate in the PinX1 knocked-down nontumorigenic breast cell. A total of 977 mRNAs and 631 lncRNAs were identified as differentially expressed transcripts between PinX1 overexpressed and control MCF-7 cells. Further analysis identified the involvement of these mRNAs in 52 cancer related pathways and various other biological processes. 11 enhancer-like lncRNAs and 25 lincRNAs with their adjacent mRNA pairs were identified as coregulated transcripts. Our results confirmed the role of PinX1 as a major tumor suppressor gene in breast cancer cell lines and provided information for further research on the molecular mechanisms of PinX1 in tumorigenesis.