Functional Analysis of VvBG1 During Fruit Development and Ripening of Grape
Functional Analysis of VvBG1 During Fruit Development and Ripening of Grape
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VvBG1在葡萄果实发育和成熟过程中的功能分析
DOI：
10.1007/s00344-016-9597-y
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发表时间：
2016-12-01
影响因子：
4.8
通讯作者：
Fang, Jinggui
中科院分区：
文献类型：
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作者：
Jia, Haifeng;Wang, Chen;Fang, Jinggui
植物生长发育过程中，β-葡萄糖苷酶（BG）通过水解脱落阿坝糖酯，释放活性阿坝（阿坝），参与ABA的合成。然而，没有遗传学证据表明基因在果实成熟过程中的作用。在这里，三个基因（VvBG 1，VvBG 2，和VvBG 3）编码β-葡萄糖苷酶的表达模式进行了分析，在葡萄果实发育过程中，它被发现，β-葡萄糖苷酶活性增加葡萄果实响应各种压力。为进一步验证β-葡萄糖苷酶在果实成熟过程中的作用，对VvBG 1基因在草莓果实中的异源表达进行了验证，结果表明VvBG 1的过表达增加了β-葡萄糖苷酶的活性，促进了草莓果实的成熟。另外，VvBG 1基因过表达可诱导草莓果实中阿坝含量增加，从而诱导果实花青苷、可溶性固形物积累和果实软化。此外，与着色（CHS，CHI，F3 H和UFGT），软化（PG 1，PL 1和EXP 1）和香气（SAAT和QR）相关的基因上调。这项工作将阐明VvBGs在阿坝合成中的具体作用，并为ABA控制葡萄成熟机制提供一些新的见解。
beta-glucosidase (BG) was believed to take part in abscisic acid (ABA) synthesis via hydrolysis of ABA glucose ester to release active ABA during plant growth and development. However, there is no genetic evidence available to indicate the role of genes during fruit ripening. Here, the expression patterns of three genes (VvBG1, VvBG2, and VvBG3) encoding beta-glucosidase were analyzed during grape fruit development, and it was found that beta-glucosidase activity increased in grape fruit in response to various stresses. Furthermore, to verify the function of beta-glucosidase during fruit ripening, heterogeneous expression of the VvBG1 gene in strawberry fruit was validated, and the results showed that the VvBG1 over-expression increased beta-glucosidase and promoted the fruit ripening process in strawberry. In addition, we found that ABA contents increased in the VvBG1 over-expression of strawberry fruit, which induced fruit anthocyanin, soluble solid accumulation, and fruit softening. Moreover, genes related to coloring (CHS, CHI, F3H, and UFGT), softening (PG1, PL1, and EXP1), and aroma (SAAT, and QR) were up-regulated. This work will elucidate the specific roles of VvBGs in the synthesis of ABA and provide some new insights into the ABA-controlled grape ripening mechanism.