Farnesol-mediated inhibition of Candida albicans yeast growth and rescue by a diacylglycerol analogue.
Farnesol-mediated inhibition of Candida albicans yeast growth and rescue by a diacylglycerol analogue.
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金合欢醇介导的白色念珠菌酵母生长抑制和二酰基甘油类似物的拯救。
DOI：
10.1002/yea.1501
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Chaffin,WLaJean
中科院分区：
文献类型：
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作者：
Uppuluri,Priya;Mekala,Satish;Chaffin,WLaJean
在白念珠菌细胞生长到早期稳定期的过程中，代谢产物在培养基中积累，包括群体感应分子法尼醇。我们发现，除抑制芽管外，40µM法尼醇也能抑制C。白念珠菌在酵母生长允许的条件下生长。与此一致的是，将酵母细胞重新悬浮在含有40µM法尼醇的新鲜培养基中进行转录分析，发现参与菌丝形成、GTP酶激活、有丝分裂和DNA复制的基因下调了许多倍。法尼醇对酵母菌生长的抑制作用依赖于菌体的生长阶段。白化细胞。添加二酰甘油类似物缓解了生长缺陷，暗示磷脂酰肌醇信号在延迟中起作用。虽然二酰甘油是哺乳动物细胞中蛋白激酶C(PKC)的激活剂，但关于真菌PKC的激活仍存在一些问题。一株缺失PKC1的突变株对法尼醇和类似野生型的甘油二酯类似物有反应，这表明PKC不是甘油二酯类似物的靶标。版权所有©2007 John Wiley&Sons，Ltd.
DuringCandida albicansyeast cell growth to early stationary phase, metabolites accumulate in the medium, including the quorum‐sensing molecule farnesol. We found that besides germ tube inhibition, 40 µMfarnesol also inhibitedC. albicansyeast growth under yeast growth permissive conditions. Consistent with this observation, transcriptional analysis of yeast cells resuspended in fresh medium with 40 µMfarnesol revealed that genes involved in hyphal formation, GTPase activation, mitosis and DNA replication were downregulated many‐fold. Farnesol‐mediated inhibition of yeast growth was dependent on the growth phase of theC. albicanscells. The growth defect was relieved by addition of a diacylglycerol analogue, implicating phosphatidylinositol signalling in the delay. Although diacylglycerol is an activator of protein kinase C (PKC) in mammalian cells, there is some question about activation of fungal PKCs. A mutant strain deleted forPKC1responded to farnesol and the diacylglycerol analogue similar to wild‐type, suggesting that PKC is not the target of the diacylglycerol analogue. Copyright © 2007 John Wiley & Sons, Ltd.