The effect of particle design on cellular internalization pathways
The effect of particle design on cellular internalization pathways
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DOI：
10.1073/pnas.0801763105
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发表时间：
2008-08-19
影响因子：
11.1
通讯作者：
DeSimone, Joseph M.
中科院分区：
文献类型：
--
作者：
Gratton, Stephanie E. A.;Ropp, Patricia A.;DeSimone, Joseph M.
已知颗粒与细胞的相互作用受到颗粒大小的强烈影响，但对颗粒大小、形状和表面化学对细胞内化和细胞内运输的相互依赖作用知之甚少。我们报告的内部化的特别设计的，单分散的水凝胶颗粒到HeLa细胞的大小，形状和表面电荷的函数。我们采用一种自上而下的颗粒制造技术，称为PRINT，能够生成均匀的有机微米和纳米颗粒，并完全控制尺寸，形状和表面化学。颗粒内化的证据是通过使用传统的生物技术和透射电子显微镜。这些研究结果表明，HeLa细胞很容易通过使用几种不同的内吞机制内化尺寸为3 μ m的非球形颗粒。此外，发现棒状颗粒在内化率方面具有明显的优势，这让人想起许多棒状细菌在非吞噬细胞中内化的优势。
The interaction of particles with cells is known to be strongly influenced by particle size, but little is known about the interdependent role that size, shape, and surface chemistry have on cellular internalization and intracellular trafficking. We report on the internalization of specially designed, monodisperse hydrogel particles into HeLa cells as a function of size, shape, and surface charge. We employ a top-down particle fabrication technique called PRINT that is able to generate uniform populations of organic micro- and nanoparticles with complete control of size, shape, and surface chemistry. Evidence of particle internalization was obtained by using conventional biological techniques and transmission electron microscopy. These findings suggest that HeLa cells readily internalize nonspherical particles with dimensions as large as 3 mu m by using several different mechanisms of endocytosis. Moreover, it was found that rod-like particles enjoy an appreciable advantage when it comes to internalization rates, reminiscent of the advantage that many rod-like bacteria have for internalization in nonphagocytic cells.