Immuno-pillar chip: a new platform for rapid and easy-to-use immunoassay
Immuno-pillar chip: a new platform for rapid and easy-to-use immunoassay
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DOI：
10.1039/c0lc00241k
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Baba, Yoshinobu
中科院分区：
文献类型：
--
作者：
Ikami, Mai;Kawakami, Ayako;Baba, Yoshinobu
我们提出了一种新的快速、易用的免疫分析芯片，我们将其命名为免疫柱芯片。它有水凝胶柱，在微通道内制造，许多抗体分子固定在1 μ m直径的聚苯乙烯珠上。为了评估芯片的性能，我们将其应用于c反应蛋白（CRP）、甲胎蛋白（AFP）和前列腺特异性抗原（PSA）、心脏和炎症标志物、肿瘤和前列腺癌标志物的夹心检测。对于疾病标志物的检测，我们确认该芯片具有分析速度快（总检测时间约为4 min）、灵敏度高、使用方便（不需要特殊技能）、样品和试剂用量小（各0.25 μ L）的特点。此外，还可以对三种生物标志物进行多重检测。此外，免疫柱芯片有一个很大的优势，即使样品或试剂的引入量不同，也几乎不会对分析结果产生任何影响。
We present a new rapid and easy-to-use immunoassay chip which we have named the immuno-pillar chip. It has hydrogel pillars, fabricated inside a microchannel, with many antibody molecules immobilized onto 1 mu m diameter polystyrene beads. To evaluate the chip performance, we applied it to the sandwich assay of C-reactive protein (CRP), alpha-fetoprotein (AFP) and prostate-specific antigen (PSA), a cardiac and inflammation marker, tumors and prostate cancer markers, respectively. For detection of disease markers, we confirmed the chip provides rapid analysis (total assay time of about 4 min) with high sensitivity, it is easy-to-use (no special skills are needed), and it uses small volumes of the sample and reagent (0.25 mu L each). Moreover, multiplex assay for three biomarkers was also possible. Additionally, the immuno-pillar chip has a big advantage of having hardly any influence on the assay results even if the introduction quantities of the sample or reagents are different.