A biomechanical, histological and biochemical study in an experimental rabbit hypospadias repair model using scanning acoustic microscopy
A biomechanical, histological and biochemical study in an experimental rabbit hypospadias repair model using scanning acoustic microscopy
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DOI：
10.1016/j.jpurol.2010.07.011
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发表时间：
2011-08-01
影响因子：
2
通讯作者：
Jorgensen, Troels Munch
中科院分区：
文献类型：
--
作者：
Lalla, Marianna;Riis, Carsten;Jorgensen, Troels Munch
目的：研究兔尿道下裂模拟修复和急诊修复后的生物力学、组织学和生物化学特性。材料与方法：38只新西兰大白兔随机分为两组，每组10只，分别行尿道下裂样修复和急诊修复(松动推进、管状切开后尿道板、改良后尿道板)和假手术。结果：松动推进组后尿路僵硬程度明显高于TIP组(P<0.001)，而TIP组明显高于其他两组(P<；0.05)。在后尿路，松动推进组和改良针尖组的胶原含量高于假手术组(P<；0.05)。腹侧尿道组间胶原含量无明显差异。结论：尿路在背侧和腹侧的不同层次上具有不同的微弹性性质，其周围结缔组织层和内部结缔组织层具有较高的硬度。修复后的尿路在长期随访时部分恢复了微米分辨率的弹性。扫描声学显微镜在显微镜水平上阐明了正常和手术后的尿路的结构和功能关系。(C)2010年《儿科泌尿外科杂志》。爱思唯尔有限公司出版。保留所有权利。
Objective: To investigate the biomechanical, histological and biochemical properties of rabbit urethra at long-term follow up after hypospadias simulation and acute repair.Materials and methods: Thirty-eight white New Zealand male rabbits underwent experimental creation of a hypospadias-like defect and acute repair (mobilization and advancement, tubularized incised posterior urethral plate (TIP), modified TIP) and sham operation. After 23 weeks all groups + controls underwent biomechanical, histological and biochemical assessments.Results: The mobilization and advancement group showed a higher stiffness compared to the TIP groups (P < 0.05) in the posterior urethra, whereas the TIP group was stiffer compared to the other two operative groups (P < 0.001) in the ventral urethra. In the dorsal urethra, the mobilization and advancement group and the modified TIP group had a higher collagen content compared to shams (P < 0.05). No differences in collagen content were found between groups in the ventral urethra. A correlation between acoustic and histological layers was found, partially related to collagen content.Conclusion: The urethras had different microelastic properties in different layers of the dorsal and ventral urethra, with higher stiffness in the connective tissue layers surrounding and within the urethra. The repaired urethras had partially recovered their elasticity at micrometer resolution at long-term follow up. Scanning acoustic microscopy elucidated structure function relationships at microscopic level in normal and operated urethra. (C) 2010 Journal of Pediatric Urology Company. Published by Elsevier Ltd. All rights reserved.