The molecular organization of collagen in saccular aneurysms assessed by polarized light microscopy.
The molecular organization of collagen in saccular aneurysms assessed by polarized light microscopy.
复制标题
通过偏光显微镜评估囊状动脉瘤中胶原蛋白的分子组织。
DOI：
10.3109/03008208808992793
复制
发表时间：
1988
影响因子：
2.9
通讯作者：
P. Canham
中科院分区：
文献类型：
--
作者：
P. Whittaker;M. E. Schwab;P. Canham
偏振光的延迟和胶原蛋白的分子组织之间的已知关系使我们能够研究脑囊状动脉瘤中的胶原蛋白，其中胶原蛋白可能是异常的结构。在尸检时获得的6个动脉瘤及其相邻动脉以7微米的厚度切片。通过de Sénarmont方法获得的苏木精和伊红染色切片中的延迟测量值在动脉瘤壁中显著低于相邻动脉的外膜区域。通过对三个动脉瘤的未染色切片进行吸渗分析，发现了类似的阻滞减少。我们认为，光学性质的差异是因为动脉瘤胶原蛋白是不成熟的，因此在分子水平上组织较少。未成熟胶原蛋白的存在也可以解释囊状动脉瘤扩大和破裂所必需的机械性质的改变。
The known relationship between the retardation of polarized light and the molecular organization of collagen enabled us to study collagen in cerebral saccular aneurysms, structures in which the collagen is probably abnormal. Six aneurysms and their adjacent arteries, obtained at autopsy, were sectioned at a thickness of 7 microns. Measurements of retardation in hematoxylin and eosin stained sections, obtained by the method of de Sénarmont, were significantly lower in the aneurysm wall compared with the adventitial region of adjacent arteries. A similar reduction in retardation was found by imbibition analysis on unstained sections from three of the aneurysms. We propose that the difference in optical properties arises because the aneurysm collagen is immature and so is less organized at the molecular level. The presence of immature collagen could also explain the altered mechanical properties that are necessary for the enlargement and rupture of saccular aneurysms.