Inactivation of endopeduncular nucleus impaired fear conditioning and hippocampal synaptic plasticity in rats
Inactivation of endopeduncular nucleus impaired fear conditioning and hippocampal synaptic plasticity in rats
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脚内核失活损害大鼠的恐惧调节和海马突触可塑性
DOI：
10.1016/j.nlm.2020.107224
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发表时间：
2020-09-01
影响因子：
2.7
通讯作者：
Zhang, Jichuan
中科院分区：
文献类型：
--
作者：
Chen, Yanmei;Kang, Na;Zhang, Jichuan
内苍白球（GPi）是基底节核团的一部分，在运动功能中起着重要作用。最近的研究表明GPi可以调节情绪加工和学习，但其可能的机制尚不清楚。本研究探讨了大鼠脚内核（EP，GPi的啮齿类同源物）对恐惧条件反射的影响。GABAA受体激动剂蝇蕈醇在大鼠恐惧条件反射前15分钟或恐惧条件反射后立即双侧进入EP。我们发现，EP失活损害大鼠恐惧记忆的获得，但不巩固。蝇蕈醇组海马CA 1区长时程增强（long-term potentiation，LTP）明显减弱，海马内丝氨酸845位点的α-氨基-3-羟基-5-甲基-4-异恶唑丙酸受体（alpha-amino-3-hydroxy-5-methyl 4-isoxazole-propionic acid receptor，AMPAR）亚单位1（GluA 1）磷酸化水平降低。结果表明，EP功能障碍可损害大鼠海马依赖性学习记忆。
The internal globus pallidus (GPi) is one part of basal ganglion nucleuses which play fundamental role in motor function. Recent studies indicated that GPi could modulate emotional processing and learning, but the possible mechanism remains still unknown. In this study, the effects of endopeduncular nucleus (EP, a rodent homolog of GPi) on fear conditioning were tested in rats. GABAA receptor agonist muscimol was bilaterally delivered into the EP 15 min before or immediately after fear conditioning in rats. We found that EP inactivation impaired the acquisition but not consolidation of fear memory in rats. Furthermore, the long-term potentiation (LTP) in hippocampal CA1 area was impaired, and the learning related phosphorylation of alpha-amino-3-hydroxy-5-methyl4-isoxazole-propionic acid receptor (AMPAR) subunit 1 (GluA1) at the Ser845 site in hippocampus was decreased in muscimol treated group. These results demonstrated that dysfunction of EP impaired hippocampal dependent learning and memory in rats.