Arsenic trioxide induces apoptosis in B-cell chronic lymphocytic leukemic cells through down-regulation of survivin via the p53-dependent signaling pathway.
Arsenic trioxide induces apoptosis in B-cell chronic lymphocytic leukemic cells through down-regulation of survivin via the p53-dependent signaling pathway.
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DOI：
10.1016/j.leukres.2013.09.019
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发表时间：
2013-12
影响因子：
2.7
通讯作者：
Zheng XL
中科院分区：
文献类型：
--
作者：
Zhang XH;Feng R;Lv M;Jiang Q;Zhu HH;Qing YZ;Bao JL;Huang XJ;Zheng XL
三氧化二砷（As_2O_3）可诱导多种肿瘤细胞凋亡。然而，相关的机制并不清楚。我们发现，As 2 O3显着抑制WSU-CLL细胞的增殖和诱导凋亡的剂量和时间依赖性的方式。经2 μM As 2 O3处理的WSU-CLL细胞，survivin表达下调，p53表达上调。Survivin siRNA联合As 2 O3进一步抑制WSU-CLL细胞的增殖。siRNA抑制p53可抑制As 2 O3对survivin的下调，并抑制As 2 O3对WSU-CLL细胞的毒性作用。提示As_2O_3对慢性淋巴细胞白血病有一定的治疗价值。
Arsenic trioxide (As2O3) can induce apoptosis in many tumors. However, the associated mechanisms are not clearly understood. We found that As2O3 significantly inhibited the proliferation of WSU-CLL cells and induced apoptosis in dose- and time-dependent manners. WSU-CLL cells treated with 2 μM As2O3 showed survivin down-regulation and p53 up-regulation. Survivin siRNA combined with As2O3 further inhibited the proliferation of WSU-CLL cells. p53 inhibition by siRNA prevented the down-regulation of survivin by As2O3 and prevented the As2O3-induced cytotoxicity of WSU-CLL cells. These results suggest that As2O3 may be of therapeutic value for chronic lymphocytic leukemia.