Generation of cell-derived matrices that support human NK cell migration and differentiation.
Generation of cell-derived matrices that support human NK cell migration and differentiation.
复制标题
支持人NK细胞迁移和分化的细胞来源矩阵。
DOI：
10.1002/jlb.1ma0420-635r
复制
发表时间：
2020-10
影响因子：
5.5
通讯作者：
Mace EM
中科院分区：
文献类型：
--
作者：
Lee BJ;Hegewisch Solloa E;Shannon MJ;Mace EM
Human NK cells are effectors of the innate immune system that originate from hematopoietic precursors in the bone marrow. While stromal cell lines that support NK cell development from hematopoietic precursors are often used to generate mature NK cells from lymphoid precursors in vitro, the nature of contributing factors of these stromal cells to the generation of functionally mature NK cells has been poorly described. Previous studies have shown that developing NK cells adhere to, and migrate on, developmentally supportive stroma. Here, we describe the generation of cell-derived matrices (CDMs) from a commonly used murine fetal liver stromal cell line. These CDMs are derived directly from the same EL08.1D2 stromal cell line known to support NK cell differentiation and contain ECM structural components fibronectin and collagen. We demonstrate that CDMs support NK cell adhesion and migration with similar properties as intact cells. Further, we show that CDMs support NK cell maturation from lymphoid precursors in vitro, albeit with reduced cell survival compared to intact cell-based differentiation. Together, these results describe a cell-free system that supports NK cell development and that can serve as a useful model for studying the nature of the biochemical interactions between NK cell developmental intermediates and developmentally supportive substrates.
