Experimental silicosis: morphologic and biochemical abnormalities produced by intratracheal instillation of quartz into guinea pig lungs.
Experimental silicosis: morphologic and biochemical abnormalities produced by intratracheal instillation of quartz into guinea pig lungs.
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实验性硅肺病：气管内将石英滴注到豚鼠肺中产生的形态和生化异常。
DOI：
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发表时间：
1980
影响因子：
6
通讯作者：
R. Daniele
中科院分区：
文献类型：
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作者：
J. Dauber;M. Rossman;G. Pietra;S. Jimenez;R. Daniele
气管内注入二氧化硅后6个月，对豚鼠肺进行组织学、超微结构、细胞学和生化研究。组织反应包括弥漫性肺泡间隔浸润伴间质纤维化和肉芽肿浸润伴结节性纤维化。超微结构研究证实肺泡上皮（组织细胞、中性粒细胞、嗜酸性粒细胞和淋巴细胞）和II型细胞增生衬里中存在混合炎性渗出物。通过灌洗回收的肺细胞数量以及支气管肺泡细胞中中性粒细胞和多核细胞的比例在实验动物中显著高于对照组（气管内盐水）（P <0.05）。实验动物的肺总胶原和培养肺组织的胶原合成也增加。由于豚鼠肺对肺内二氧化硅的反应包括人类矽肺和特发性肺纤维化常见的病理特征，因此该模型具有提高我们对这两种重要临床疾病的理解的潜力。
Six months after intratracheal instillation of silica, histologic, ultrastructural, cytologic, and biochemical studies were performed on the lungs of guinea pigs. The tissue response consisted of both diffuse alveolar septal infiltration with interstitial fibrosis and granulomatous infiltration with nodular fibrosis. Ultrastructural studies confirmed the presence of a mixed inflammatory exudate in the alveolar interstitium (histiocytes, neutrophils, eosinophils, and lymphocytes) and the Type II lining of cell hyperplasia. The number of lung cells recovered by lavage and the proportions of neutrophils and multinucleated cells in bronchoalveolar cells were significantly greater in experimental animals (P < .05) than in controls (intratracheal saline). Total lung collagen and collagen synthesis by cultured lung tissue were also increased in the experimental animals. Since the response of guinea pig lung to intratracheal silica included pathologic features common to human silicosis and idiopathic pulmonary fibrosis, this model has the potential for improving our understanding of both of these important clinical disorders.