Axonal transport of rubidium and thallium in the olfactory nerve of mice
Axonal transport of rubidium and thallium in the olfactory nerve of mice
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DOI：
10.1016/j.nucmedbio.2005.03.009
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发表时间：
2005-07-01
影响因子：
3.1
通讯作者：
Amano, R
中科院分区：
文献类型：
--
作者：
Kanayama, Y;Enomoto, S;Amano, R
在小鼠鼻腔注射放射性Rb-86(+)和TL-201(+)后，我们观察到这种通过嗅神经通路的直接运输。将(RbcL)-Rb-86和(TICl)-T-201溶液分成单侧鼻腔给药组和静脉给药组。处死后，将其头部分为左右两侧，再分成7个部分，分离鼻黏膜和脑区。单侧鼻腔给药后6h，同侧嗅球的摄取(Rb-86，0.7%剂量；TL-201，0.5%剂量)显著高于对侧(Rb-86，0.08%剂量；TL-201，0.15%剂量)。此外，在嗅球中积聚的Rb-86和TL-201逐渐输送到嗅束的其他脑区、与鼻孔对应的一侧的端脑和间脑。给药后6h和12h左右脑区差异有统计学意义。此外，TL-201放射自显影清楚地显示密集堆积的条状图案，位于嗅球的肾小球层和颗粒细胞层周围以及嗅皮层周围。这些结果为轴突通过嗅神经通路，从鼻腔到嗅球，以及通过突触连接到嗅皮层提供了明确的证据。RB-86(+)和TL-201(+)的嗅觉转运被认为代表了K+在嗅觉系统中的行为。(C)2005 Elsevier Inc.保留所有权利。
Following intranasal administration of radioactive Rb-86(+) and Tl-201(+) in mice, we observed this direct transport via the olfactory nerve pathway. The (RbCl)-Rb-86 and (TICl)-T-201 solutions were administered to two groups of mice, the unilateral intranasal and intravenous administration groups. After sacrifice, their heads were divided into the right and left side, which were then subdivided into seven parts; the nasal mucosa and brain regions were separated. Following the unilateral intranasal administration, uptake after 6 It by the olfactory bulb was significantly higher on the ipsilateral side (Rb-86, 0.7 %dose; Tl-201, 0.5 %dose) than on the contralateral side (Rb-86, 0.08 %dose; Tl-201, 0.15 %dose). Moreover, the Rb-86 and Tl-201 that accumulated in the olfactory bulb were gradually transported to other brain regions of the olfactory tract, the telencephalon and the diencephalon on the side corresponding to the nostril used for administration. Significant differences were observed between the right and left side of the brain regions 6 and 12 h after administration. Further, Tl-201 autoradiography clearly showed striped patterns of dense accumulation, localized in the region around the glomerular layer and granule cell layer of the olfactory bulb and around the olfactory cortex. These results provide clear evidence of axonal transport via the olfactory nerve pathway, from nasal cavity to the olfactory bulb, as well as to the olfactory cortex through the synaptic junctions. The olfactory transport of the Rb-86(+) and Tl-201(+) is thought to represent the behavior of K+ in the olfactory system. (c) 2005 Elsevier Inc. All rights reserved.