EFFECTS OF HALOTHANE AND OTHER VOLATILE ANESTHETICS ON PROTEIN-SYNTHESIS AND SECRETION IN GUINEA-PIG LIVER SLICES
EFFECTS OF HALOTHANE AND OTHER VOLATILE ANESTHETICS ON PROTEIN-SYNTHESIS AND SECRETION IN GUINEA-PIG LIVER SLICES
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DOI：
10.1093/bja/68.2.172
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发表时间：
1992-02-01
影响因子：
9.8
通讯作者：
BRENDEL, K
中科院分区：
文献类型：
--
作者：
GHANTOUS, HN;FERNANDO, JL;BRENDEL, K
我们研究了挥发性麻醉剂对哈特利雄性豚鼠肝脏切片中蛋白质合成和分泌的影响。 将切片（250-300 μm 厚）在含有 Krebs-Henseleit 缓冲液的密封滚瓶中在 37 摄氏度、95% 氧气下孵育。 挥发性麻醉剂在小瓶中蒸发，在培养基中产生恒定浓度。 氟烷 1-2.1 mmol litre-1 会导致蛋白质合成（H-3-亮氨酸掺入）和分泌出现浓度相关的下降。 氘代氟烷（d-氟烷）的生物转化程度较低，其抑制性比氟烷低：H-3-亮氨酸的摄取不受影响，但其与新生肽的结合受到抑制。 安氟烷 2.2 mmol litre-1、异氟烷 2.2 mmol litre-1 和七氟烷 2.1 mmol litre-1 也抑制蛋白质合成，但程度低于氟烷和 d-氟烷。 我们的结论是，蛋白质合成和分泌的改变是肝切片中挥发性麻醉剂造成的细胞损伤的早期且敏感的指标。
We have investigated the effect of volatile anaesthetics on protein synthesis and secretion in Hartley male guineapig liver slices. The slices (250-300-mu-m thick) were incubated in sealed roller vials containing Krebs-Henseleit buffer at 37-degrees-C under 95% oxygen. Volatile anaesthetics were vaporized in the vials to produce constant concentrations in the medium. Halothane 1-2.1 mmol litre-1 produced a concentration-related decrease in protein synthesis (H-3-leucine incorporation) and secretion. Deuterated halothane (d-halothane), which is less biotransformed, was less inhibiting than halothane: uptake of the H-3-leucine was not affected but its incorporation into the nascent peptide was inhibited. Enflurane 2.2 mmol litre-1, isoflurane 2.2 mmol litre-1 and sevoflurane 2.1 mmol litre-1 also inhibited protein synthesis, but to a lesser extent than halothane and d-halothane. We conclude that alterations in protein synthesis and secretion are an early and sensitive indicator of cellular injury by volatile anaesthetics in liver slices.