Localization of superoxide anion in the red tide alga Chattonella antiqua.
Localization of superoxide anion in the red tide alga Chattonella antiqua.
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赤潮藻 Chattonella antiqua 中超氧阴离子的定位。
DOI：
10.1177/41.4.8383714
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
M. Watanabe
中科院分区：
文献类型：
--
作者：
M. Shimada;S. Kawamoto;Y. Nakatsuka;M. Watanabe
为了研究Chattonella antiqua赤潮产生的超氧阴离子的定位，我们在培养液中加入了3，7-dihydro-6-[4-（2-（N '-（5-fluoresceinyl）thioureido）ethoxy）phenyl]-2-methylimidazo（1，2-a）pyrazin-3-one（FCLA），FCLA是一种Cypridina antiqua的类似物，也是一种检测O2-和1 O2的特异性荧光探针。用激光扫描显微镜观察了活体植物病原菌。在高倍镜下（x 6400），发现细胞表面有许多大小不一的疣状突起。当用氩激光（488 nm）照射细胞时，在每个突起处注意到非常强的荧光。这种荧光被完全抑制的超氧化物歧化酶加入到孵育培养基中，从而表明O2-生成在每个疣状突起的细胞表面上。
To investigate the localization of superoxide anion generated in the red tide alga Chattonella antiqua, we added to the incubation medium 3,7-dihydro-6-[4-(2-(N'-(5-fluoresceinyl)thioureido) ethoxy)phenyl]-2-methylimidazo(1,2-a)pyrazin-3-one (FCLA), which is a Cypridina luciferin analogue and a specific fluorescent probe for detecting O2- and 1O2. The live phytoplankter was observed with the laser scanning microscope. At high magnification (x 6400) it was found that the plankter has many large and small verruciform protrusions on the cell surface. When the cell was irradiated with an argon laser (488 nm), a very intense fluorescence was noted at each protrusion. This fluorescence was completely inhibited by the addition of superoxide dismutase to the incubation medium, thus suggesting O2- generation at each verruciform protrusion on the cell surface.