Neuropilin receptors guide distinct phases of sensory and motor neuronal segmentation
Neuropilin receptors guide distinct phases of sensory and motor neuronal segmentation
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DOI：
10.1242/dev.032920
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发表时间：
2009-06-01
期刊：
影响因子：
4.6
通讯作者：
Gammill, Laura S.
中科院分区：
文献类型：
--
作者：
Roffers-Agarwal, Julaine;Gammill, Laura S.
节段主干周围神经系统是由腹侧迁移的神经嵴细胞产生的，这些细胞只侵入前硬膜，并分化为背根和交感神经节。同时，腹侧脊髓运动轴突也以节段方式通过体突投射。周围神经系统分割是如何产生的尚不清楚。我们之前的研究表明，神经匹素2 (Nrp2)/信号蛋白3F （Sema3F）信号是节段性神经嵴迁移所必需的，但不是特异背根神经节发生所必需的。我们现在扩展了这些结果，表明Nrp2也参与了初始运动轴突的生长。随后，Nrp1/Sema3A信号对于节段性背根神经节的形成和运动轴突向腹根的束控是必不可少的。引人注目的是，Nrp/Sema信号不是交感神经节分割所必需的。这些数据表明Nrp2和Nrp1共同作用产生感觉神经和运动神经的分割，并且背周围神经系统的分节是在一个逐步的、依赖于nrp的过程中产生的。
The segmented trunk peripheral nervous system is generated by ventrally migrating neural crest cells that exclusively invade the anterior sclerotome and differentiate into metameric dorsal root and sympathetic ganglia. Meanwhile, ventral spinal motor axons also project through the somites in a segmental fashion. How peripheral nervous system segmentation is generated is unknown. We previously showed that neuropilin 2 (Nrp2)/semaphorin 3F (Sema3F) signaling is required for segmental neural crest migration, but not for metameric dorsal root gangliogenesis. We now expand these results to show that Nrp2 patterns initial motor axon outgrowth as well. Later, Nrp1/Sema3A signaling is essential for segmental dorsal root gangliogenesis and motor axonal fasciculation into ventral roots. Strikingly, Nrp/Sema signaling is not required for sympathetic ganglia segmentation. These data show that Nrp2 and Nrp1 work together to produce segmentation of sensory and motor nerves, and that dorsal peripheral nervous system metamerism is generated in a stepwise, Nrp-dependent process.