GLUTAMATE INDUCES A DEPOLARIZATION OF ADULT-RAT DORSAL-ROOT GANGLION NEURONS THAT IS MEDIATED PREDOMINANTLY BY NMDA RECEPTORS
GLUTAMATE INDUCES A DEPOLARIZATION OF ADULT-RAT DORSAL-ROOT GANGLION NEURONS THAT IS MEDIATED PREDOMINANTLY BY NMDA RECEPTORS
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DOI：
10.1016/0304-3940(88)90037-7
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发表时间：
1988-12-05
影响因子：
2.5
通讯作者：
WEIGHT, FF
中科院分区：
文献类型：
--
作者：
LOVINGER, DM;WEIGHT, FF
采用全细胞膜片钳技术，研究了谷氨酸、谷氨酸受体激动剂和影响谷氨酸反应的物质对分离自成年大鼠背根神经节的神经元的作用。在电流钳中，谷氨酸（100 μ M）或兴奋性氨基酸受体激动剂N-甲基-D-天冬氨酸（NMDA; 10或100 μ M）诱导膜去极化。在电压钳下，这些化合物在Mg 2+存在的情况下诱导了电压依赖性的内向电流。NMDA诱导的电流被NMDA受体拮抗剂D，L-2-氨基-5-膦酰基-戊酸酯（APV; 100 μ M）抑制，并被100 nM甘氨酸增强。很少有神经元对受体激动剂红藻氨酸盐（100 μ M）或使君子酸盐（1-10 μ M）有反应。这些观察结果表明，谷氨酸诱导的初级感觉神经元的去极化主要是由NMDA受体介导的。
The effects of glutamate, glutamate receptor agonists and substances that affect glutamate responses were studied, using the whole-cell patch-clamp technique, on neurons isolated from adult rat dorsal root ganglia. In current-clamp, glutamate (100 .mu.M) or the excitatory amino acid receptor agonist N-methyl-D-aspartate (NMDA; 10 or 100 .mu.M) induced membrane depolarization. Under volatage-clamp, these compounds induced an inward current that was voltage-dependent in the presence of Mg2+. The NMDA-induced current was inhibited by the NMDA receptor antagonist D,L-2-amino-5-phosphono-valerate (APV; 100 .mu.M), and potentiated by 100 nM glycine. Few neurons responded to the responded to the receptor agonists kainate (100 .mu.M), or quisqualate (1-10 .mu.M). These observations suggest that glutamate-induced depolarization of primary sensory neurons is mediated predominantly by NMDA receptors.