Circumferential Strain Can Be Used to Detect Lipopolysaccharide-Induced Myocardial Dysfunction and Predict the Mortality of Severe Sepsis in Mice.
Circumferential Strain Can Be Used to Detect Lipopolysaccharide-Induced Myocardial Dysfunction and Predict the Mortality of Severe Sepsis in Mice.
复制标题
周向应变可用于检测脂多糖诱导的心肌功能障碍并预测小鼠严重脓毒症的死亡率
DOI：
10.1371/journal.pone.0155346
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Xu D
中科院分区：
文献类型：
--
作者：
Chu M;Gao Y;Zhou B;Wu B;Wang J;Xu D
脓毒症引起的心肌功能障碍是脓毒症休克常见且严重的并发症。然而，常规超声心动图往往不能显示严重败血症的心肌抑制。近年来，基于斑点跟踪超声心动图（STE）的应变测量已被用于评估心功能。目的探讨STE在检测脂多糖（LPS）诱导的心功能障碍中的作用，采用m型超声心动图和二维超声心动图对LPS治疗小鼠进行检测。方法分别给予LPS 10mg/kg （n = 10）、20mg/kg （n = 10）、25mg/kg （n = 30）诱导心功能障碍。随后，用标准m型示踪仪测量了弹射分数（EF）和分数缩短（FS），而用STE测量了周向应变（Scirc）和径向应变（Srad）。通过血清生化和心脏组织病理学检查评估脓毒症引起的心肌损伤。结果血清生化和组织学检查显示，20mg/kg LPS加重心肌恶化、心肌损伤和心肌收缩功能障碍。在常规超声心动图上，10mg/kg和20mg/kg LPS组的射血分数（EF）和分数缩短（FS）相似，而20mg/kg LPS组的Scirc减少，但Srad没有减少。此外，注射LPS后20 h Scirc与小鼠体温呈正相关（r = 0.746, p = 0.001），而Srad与体温无显著相关（r = 0.356, p = 0.123）。此外，与低剂量组（-2%至-5.9%）相比，高Scirc（-5.9%至-10.4%）小鼠在给予25mg/kg LPS后死亡率降低（p = 0.03）。综上所述，我们的研究结果表明，环周应变是评估lps诱导小鼠心功能障碍的一个特异性和可靠的指标。
Background Sepsis-induced myocardial dysfunction is a common and severe complication of septic shock. However, conventional echocardiography often fails to reveal myocardial depression in severe sepsis. Recently, strain measurements based on speckle tracking echocardiography (STE) have been used to evaluate cardiac function. Aims To investigate the role of STE in detecting lipopolysaccharide (LPS)-induced cardiac dysfunction, M-mode and 2-D echocardiography were used in LPS-treated mice. Methods The mice were treated with a 10mg/kg (n = 10), 20mg/kg (n = 10) or 25mg/kg LPS (n = 30) to induce cardiac dysfunction. Subsequently, the ejection fraction (EF) and fractional shortening (FS) were measured with standard M-mode tracings, whereas the circumferential (Scirc) and radial strain (Srad) were measured with STE. Serum biochemical and cardiac histopathological examinations were performed to assess sepsis-induced myocardial injury. Results 20mg/kg LPS resulted in more deterioration, myocardial damage and cardiac contractile dysfunction based on serum biochemical and histological examinations. The mice that were subjected to 20mg/kg LPS exhibited reduced Scirc but no reduction in Srad, whereas on conventional echocardiography, the ejection fraction (EF) and fractional shortening (FS) were similar in the 10mg/kg and 20mg/kg groups. Moreover, Scirc was positively correlated with body temperature in the mice at 20 h after LPS injection (r = 0.746, p = 0.001), but no significant correlation was observed between Srad and body temperature (r = 0.356, p = 0.123). Moreover, the mice with high Scirc (-5.9% to -10.4%) exhibited reduced mortality following the administration of 25mg/kg LPS (p = 0.03) compared with the low-strain group (-2% to -5.9%). Conclusions Taken together, our findings indicate that circumferential strain is a specific and reliable indicator for evaluating LPS-induced cardiac dysfunction in mice.