Lipid Vesicle-Coated Complex Coacervates
Lipid Vesicle-Coated Complex Coacervates
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DOI：
10.1021/acs.langmuir.9b00213
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发表时间：
2019-06-18
期刊：
影响因子：
3.9
通讯作者：
Keating, Christine D.
中科院分区：
文献类型：
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作者：
Cakmak, Fatma Pir;Grigas, Alex T.;Keating, Christine D.
通过复凝聚进行的区室化在一系列不同的领域中是重要的，包括亚细胞和益生元组织、生物医学、食品科学和个人护理产品。通常，脂质自组装体如囊泡也存在于细胞内或商业制剂中。系统地了解磷脂囊泡如何与不同的复合凝聚体相互作用，可以提供洞察力，并提高对这些系统的控制。在这篇手稿中，阴离子磷脂囊泡被添加到一系列不同的复合凝聚层样品中，其中凝聚层是通过将五种聚阳离子之一与三种化学结构和长度不同的（聚）阴离子之一混合而形成的。发现囊泡在凝聚层/连续相界面处组装和/或形成聚集体。我们报告的因素，如聚电解质的电荷密度和阳离子与阴离子部分的电荷比如何影响凝聚样品中的囊泡分布。我们的研究结果强调了囊泡和聚阳离子之间的相互作用的重要性，在稀释的上清液相，以确定囊泡聚集之前，在液-液界面组装。还研究了RNA寡核苷酸（A（15））的摄取，以了解这些脂质体涂层对扩散到凝聚液滴中的影响。其中均匀的囊泡冠围绕凝聚液滴组装而不限制生物分子（如RNA）的进入的系统可以作为生物反应器。
Compartmentalization by complex coacervation is important across a range of different fields including subcellular and prebiotic organization, biomedicine, food science, and personal care products. Often, lipid self assemblies such as vesicles are also present intracellularly or in commercial formulations. A systematic understanding of how phospholipid vesicles interact with different complex coacervates could provide insight and improve control over these systems. In this manuscript, anionic phospholipid vesicles were added to a series of different complex coacervate samples in which coacervates were formed by mixing one of five polycations with one of three (poly)anions that varied in chemical structure and length. Vesicles were found to assemble at the coacervate/continuous phase interface and/or form aggregates. We report how factors such as the charge density of polyelectrolytes and the charge ratio of cationic-to-anionic moieties impact the vesicle distribution in coacervate samples. Our findings emphasize the importance of interactions between vesicles and polycations in the dilute supernatant phase for determining whether the vesicles aggregate prior to assembly at the liquid liquid interface. The uptake of an RNA oligonucleotide (A(15)) was also investigated to understand the effect of these liposome coatings on diffusion into coacervate droplets. Systems in which uniform vesicle coronas assemble around coacervate droplets without restricting the entry of biomolecules such as RNAs could be of interest as bioreactors.