Structures of Sugar Chains of Human Kidney γ-Glutamyltranspeptidase
Structures of Sugar Chains of Human Kidney γ-Glutamyltranspeptidase
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人肾γ-谷氨酰转肽酶的糖链结构
DOI：
10.1093/oxfordjournals.jbchem.a135479
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发表时间：
1986
影响因子：
2.7
通讯作者：
A. Kobata
中科院分区：
文献类型：
--
作者：
K. Yamashita;A. Hitoi;Y. Matsuda;T. Miura;N. Katunuma;A. Kobata
从人肾脏纯化的γ-谷氨酰转肽酶在每个分子中含有4-5个天冬酰胺连接的糖链。糖链通过肼解从酶的多肽部分释放为低聚糖，并通过纸电泳分离为一个中性部分和两个酸性部分。通过连续的外切糖苷酶消化和甲基化分析，中性部分（占总寡糖的69%）显示为二分的双触角和三触角复合型糖链的混合物，在近端N-乙酰葡糖胺残基上有和没有岩藻糖，在其外链部分中有Gal β 1-4GlcNAc和/或Gal β 1-4（Fuc α 1-3）GlcNAc基团。酸性寡糖级分是在其外链部分中具有Gal β 1-4GlcNAc和/或Gal β 1-4（Fuc α 1-3）GlcNAc基团的二等分三触角复合型寡糖的单唾液酸衍生物和二唾液酸衍生物的混合物。酸性寡糖的一些外链被认为是唾液酸化的X抗原结构。
gamma-Glutamyltranspeptidase purified from human kidneys contains 4-5 asparagine-linked sugar chains in each molecule. The sugar chains were released from the polypeptide portion of the enzyme by hydrazinolysis as oligosaccharides and separated by paper electrophoresis into one neutral and two acidic fractions. By sequential exoglycosidase digestion and methylation analysis, the neutral fraction, which comprised 69% of total oligosaccharides, was shown to be a mixture of bisected bi- and triantennary complex-type sugar chains with and without a fucose on the proximal N-acetylglucosamine residue and with Gal beta 1----4GlcNAc and/or Gal beta 1----4(Fuc alpha 1----3)GlcNAc groups in their outer chain moieties. The acidic oligosaccharide fractions were mixtures of mono- and disialyl derivatives of bisected triantennary complex-type oligosaccharides with Gal beta 1----4GlcNAc and/or Gal beta 1----4(Fuc alpha 1----3)GlcNAc group in their outer chain moieties. Some of the outer chains of the acidic oligosaccharides were considered to be sialylated X-antigenic structures.