Analysis of the interactions of sulfur-containing amino acids in membrane proteins
Analysis of the interactions of sulfur-containing amino acids in membrane proteins
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DOI：
10.1002/pro.2955
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发表时间：
2016-08-01
期刊：
影响因子：
8
通讯作者：
Pardo, Leonardo
中科院分区：
文献类型：
--
作者：
Gomez-Tamayo, Jose C.;Cordomi, Arnau;Pardo, Leonardo
与芳香族-芳香族、芳香族-脂肪族或/和脂肪族-脂肪族相互作用相比，Met和Cys与其他氨基酸侧链的相互作用很少受到关注。准确地说，这些是唯一含有硫原子的氨基酸，硫原子具有高度极化性，因此可能参与强范德华相互作用。对膜蛋白晶体结构中存在的相互作用的分析，以及从头开始水平上在小分子模型系统中表征它们的强度，预测Met-Met相互作用强于Met-Cys，近似于Met-Phe，近似于Cys-Phe相互作用，强于Phe-Phe，近似于Phe-Leu相互作用，强于Met-Leu相互作用，强于Leu-Leu，近似于Cys-Leu相互作用。这些结果表明，含硫氨基酸形成比芳香族或脂肪族氨基酸更强的相互作用。因此，这些氨基酸可以为维持膜蛋白的3D结构提供额外的驱动力，并且可以提供功能特异性。
The interactions of Met and Cys with other amino acid side chains have received little attention, in contrast to aromatic-aromatic, aromatic-aliphatic or/and aliphatic-aliphatic interactions. Precisely, these are the only amino acids that contain a sulfur atom, which is highly polarizable and, thus, likely to participate in strong Van der Waals interactions. Analysis of the interactions present in membrane protein crystal structures, together with the characterization of their strength in small-molecule model systems at the ab-initio level, predicts that Met-Met interactions are stronger than Met-Cys approximate to Met-Phe approximate to Cys-Phe interactions, stronger than Phe-Phe approximate to Phe-Leu interactions, stronger than the Met-Leu interaction, and stronger than Leu-Leu approximate to Cys-Leu interactions. These results show that sulfur-containing amino acids form stronger interactions than aromatic or aliphatic amino acids. Thus, these amino acids may provide additional driving forces for maintaining the 3D structure of membrane proteins and may provide functional specificity.