The Spatial and Temporal Characters of Demyelination and Remyelination in the Cuprizone Animal Model
The Spatial and Temporal Characters of Demyelination and Remyelination in the Cuprizone Animal Model
复制标题
Cuprizone动物模型中脱髓鞘和髓鞘再生的时空特征
DOI：
10.1002/ar.24216
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发表时间：
2019-11
期刊：
影响因子：
--
通讯作者：
Jianmei Ma
中科院分区：
文献类型：
--
作者：
Yanli Zhang;Lin Cai;Kai Fan;Bin Fan;Ning Li;Wenting Gao;Xiaohan Yang;Jianmei Ma
多发性硬化症（MS）是年轻人中最常见的中枢神经系统疾病，由于脱髓鞘，目前还没有治愈方法。产生一些实验动物模型以模拟MS病理特征的特定方面。其中，cuprizone（CPZ）诱导的小鼠脱髓鞘模型呈现出局灶性和弥漫性病变的异质性病理。考虑到MS是一种进行性疾病，研究MS动物模型中脱髓鞘和髓鞘再生的时空特征非常重要。然而，这些数据，特别是在一些脑区，如外侧隔区，海马伞，海马仍然缺乏。在这项研究中，我们调查了髓鞘在这些领域的变化，并行胼胝体冠状面的变化。我们发现，在C57 BL/6 J小鼠与CPZ治疗1至5周的不同脑区的脱髓鞘的进展不同。这一结果表明，每个大脑区域对CPZ中毒具有不同的敏感性。有趣的是，活化的小胶质细胞不仅出现在活动性脱髓鞘区域，而且出现在非髓鞘溶解区域。停药后，胼胝体早在3天就开始有明显的髓鞘再生。整个大脑区域的髓鞘再生需要3周时间完成。我们的研究详细描述了CPZ模型脑中髓鞘改变和小胶质细胞状态的动态特征。这一信息是有价值的，以促进进一步的MS研究利用CPZ模型。Anat Rec，302：2020-2029，2019.© 2019美国解剖学协会
Multiple sclerosis (MS) is the most common central nervous system disease due to demyelination in young adults, and currently, there is no cure. Some experimental animal models were generated to mimic specific aspects of MS pathological characteristics. Among them, the cuprizone (CPZ)‐induced mouse demyelination model presents heterogeneous pathologies with both focal and diffuse lesions. Considering that MS is a progressive disease, it is important to study the spatial and temporal characters of de‐ and remyelination in MS animal models. However, such data especially in some brain regions such as lateral septal area, fimbria of hippocampus, and hippocampus are still lacking. In this study, we investigated the alterations of myelin in these areas in parallel to the changes in corpus callosum using coronal sections. We found that the progression of demyelinating varied in different brain regions in C57BL/6J mice treated with CPZ for 1 to 5 weeks. This result suggests that each brain region has a distinct sensitivity to CPZ intoxication. Interestingly, activated microglia appeared not only in the active demyelinating areas but also in the non‐myelinolysis regions. After CPZ withdrawal, significant remyelination was started in corpus callosum as early as 3 days. The completion of remyelination in the entire brain regions took 3 weeks. Our study detailed characterized the dynamics of myelin alterations and microglial status in the brain of the CPZ model. This information is valuable to facilitate further MS studies utilizing the CPZ model. Anat Rec, 302:2020–2029, 2019. © 2019 American Association for Anatomy
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DOI：
10.1016/b978-0-12-813014-8.00016-0
发表时间：
2019
期刊：
Handbook of Sleep Disorders in Medical Conditions
影响因子：
--
作者：
C. Veauthier;F. Paul
通讯作者：
C. Veauthier;F. Paul
DOI：
10.1097/nen.0b013e3181e3de90
发表时间：
2010-07
影响因子：
3.2
作者：
Xie M;Tobin JE;Budde MD;Chen CI;Trinkaus K;Cross AH;McDaniel DP;Song SK;Armstrong RC
通讯作者：
Armstrong RC
影响因子：
6.7
作者：
Kipp, M.;Norkus, A.;Krauspe, B.;Clarner, T.;Berger, K.;van der Valk, P.;Amor, S.;Beyer, C.
通讯作者：
Beyer, C.
DOI：
10.1523/jneurosci.23-30-09824.2003
发表时间：
2003-10
期刊：
The Journal of Neuroscience
影响因子：
--
作者：
H. Arnett;Ying Wang;G. Matsushima;Kinuko Suzuki;J. Ting
通讯作者：
H. Arnett;Ying Wang;G. Matsushima;Kinuko Suzuki;J. Ting
影响因子：
3.1
作者：
Goldberg, Johannes;Clarner, Tim;Kipp, Markus
通讯作者：
Kipp, Markus