TRANSCRIPTIONAL CONTROL IN THE PRODUCTION OF LIVER-SPECIFIC MESSENGER-RNAS
TRANSCRIPTIONAL CONTROL IN THE PRODUCTION OF LIVER-SPECIFIC MESSENGER-RNAS
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DOI：
10.1016/0092-8674(81)90436-0
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
DARNELL, JE
中科院分区：
文献类型：
--
作者：
DERMAN, E;KRAUTER, K;DARNELL, JE
制备与[小鼠]肝脏mRNA互补的c[互补]DNA克隆，并用于测定来自肝脏、脑和肝癌细胞的分离细胞核中特定基因的转录速率。与仅（或主要）在肝脏中发现的mRNA互补的cDNA序列仅与来自肝细胞核的标记的核RNA杂交。看来，转录事件是主要负责这些，也许大多数，组织特异性中等丰度的mRNA的合成。
c[complementary]DNA clones complementary to [mouse] liver mRNA were prepared and used to determine transcription rates of specific genes in isolated nuclei from liver, brain and hepatoma cells. The cDNA sequences complementary to mRNA found only (or mainly) in the liver hybridize to labeled nuclear RNA only from liver nuclei. It appears that transcriptional events are primarily responsible for the synthesis of these, and perhaps most, tissue-specific moderately abundant mRNA.