Refining the Quality of Monoclonal Antibodies: Grafting Unique Peptide-Binding Site in the Fab Framework.
Refining the Quality of Monoclonal Antibodies: Grafting Unique Peptide-Binding Site in the Fab Framework.
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提高单克隆抗体的质量：在 Fab 框架中嫁接独特的肽结合位点。
DOI：
10.1007/978-1-4939-8958-4_12
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Williams,JohnC
中科院分区：
文献类型：
--
作者：
King,JeremyD;Williams,JohnC
单抗是一种主要的治疗方法。将表位结合位点嫁接到单抗上，也称为表位使能，可以在不改变稳定性或抗原结合的情况下，通过提供额外的蛋白质-蛋白质相互作用表面来扩展单抗的用途。我们以前曾使用这个位置来连接染料、细胞毒性药物和整个蛋白质。在这里，我们提供了一种简单的方法，用于使能冥想表位的mAbs，并使用流式细胞术(FACS)验证冥想表位和抗原结合。
Monoclonal antibodies (mAbs) are a major therapeutic modality. Grafting the meditope binding site onto mAbs, also known as meditope-enabling, can extend the usefulness of mAbs by providing an additional protein-protein interaction surface without altering the stability or antigen binding. We have previously used this site for attaching dyes, cytotoxic drugs, and entire proteins. Here, we provide a simple protocol for meditope-enabling mAbs, and verifying meditope and antigen binding using flow cytometry (FACS).