Dissemination of Mycobacterium tuberculosis is influenced by host factors and precedes the initiation of T-cell immunity
Dissemination of Mycobacterium tuberculosis is influenced by host factors and precedes the initiation of T-cell immunity
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DOI：
10.1128/iai.70.8.4501-4509.2002
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发表时间：
2002-08-01
影响因子：
3.1
通讯作者：
Behar, SM
中科院分区：
文献类型：
--
作者：
Chackerian, AA;Alt, JM;Behar, SM
我们报告结核分枝杆菌在小鼠中的传播是在宿主控制下的，并且先于t细胞免疫的启动。气溶胶接种后9至11天，结核分枝杆菌传播到肺淋巴结（LN）， 2至3天后在那里检测到结核分枝杆菌特异性T细胞。这表明细菌的最初传播是通过淋巴引流发生的，获得性t细胞免疫反应是在引流的淋巴中产生的。在感染后11至14天传播到周围部位，如脾脏和肝脏，随后在肺和脾脏出现结核分枝杆菌特异性T细胞。在所研究的所有病例中，扩散到LN或脾脏先于该器官中结核分枝杆菌特异性T细胞的激活。有趣的是，细菌从耐药C57BL/6小鼠的肺部传播比从易感C3H小鼠的肺部传播更早，因此，C57BL/6小鼠比C3H小鼠更早产生对结核分枝杆菌的免疫反应。因此，结核分枝杆菌的早期传播可能有助于启动适当和及时的免疫反应，而不是传播感染。我们推测，这种接种结核分枝杆菌后的早期免疫可能有助于C57BL/6小鼠的优越抗性。
We report that dissemination of Mycobacterium tuberculosis in the mouse is under host control and precedes the initiation of T-cell immunity. Nine to eleven days after aerosol inoculation, M. tuberculosis disseminates to the pulmonary lymph nodes (LN), where M. tuberculosis-specific T cells are detected 2 to 3 days thereafter. This indicates that the initial spread of bacteria occurs via lymphatic drainage and that the acquired T-cell immune response is generated in the draining LN. Dissemination to peripheral sites, such as the spleen and the liver, occurs 11 to 14 days postinfection and is followed by the appearance of M. tuberculosis-specific T cells in the lung and the spleen. In all cases studied, dissemination to the LN or the spleen preceded activation of M. tuberculosis-specific T cells in that organ. Interestingly, bacteria disseminate earlier from the lungs of resistant C57BL/6 mice than from the lungs of susceptible C3H mice, and consequently, C57BL/6 mice generate an immune response to M. tuberculosis sooner than C3H mice generate an immune response. Thus, instead of spreading infection, early dissemination of M. tuberculosis may aid in the initiation of an appropriate and timely immune response. We hypothesize that this early initiation of immunity following inoculation with M. tuberculosis may contribute to the superior resistance of C57BL/6 mice.