Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions
Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions
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DOI：
10.1242/dev.051011
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发表时间：
2010-08
期刊：
影响因子：
4.6
通讯作者：
Mai Yamamoto;Ryoko Morita;Takamasa Mizoguchi;Hiromi Matsuo;Miho Isoda;Tohru Ishitani;A. Chitnis;Kunihiro Matsumoto;J. G. Crump;K. Hozumi;S. Yonemura;K. Kawakami;Motoyuki Itoh
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文献类型：
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作者：
Mai Yamamoto;Ryoko Morita;Takamasa Mizoguchi;Hiromi Matsuo;Miho Isoda;Tohru Ishitani;A. Chitnis;Kunihiro Matsumoto;J. G. Crump;K. Hozumi;S. Yonemura;K. Kawakami;Motoyuki Itoh
在发育中的胚胎中，细胞-细胞信号传导是组织模式和结构组织所必需的。在中线发育过程中，脊索在形成轴向结构的同时对其周围组织的形成起作用；然而，这些模式和结构角色如何协调仍然是难以捉摸的。在这里，我们确定了Notch信号调节脊索模式活动和结构完整性的机制。我们发现Mind bomb （Mib）使Jagged 1 （Jag1）泛素化，并且在信号发射细胞中对Jag1激活Notch信号至关重要。在斑马鱼中，功能丧失和功能获得分析表明，Mib-Jag1-Notch信号有利于非空泡细胞的发育，而牺牲了脊索中空泡细胞的发育。这导致脊索周围基底膜的形成和周围肌肉先驱细胞的模式发生变化。这些数据揭示了一种以前未被认识的机制，通过Mib-Jag1-Notch信号介导的细胞命运决定来调节脊索的模式和结构作用。
In the developing embryo, cell-cell signalling is necessary for tissue patterning and structural organization. During midline development, the notochord plays roles in the patterning of its surrounding tissues while forming the axial structure; however, how these patterning and structural roles are coordinated remains elusive. Here, we identify a mechanism by which Notch signalling regulates the patterning activities and structural integrity of the notochord. We found that Mind bomb (Mib) ubiquitylates Jagged 1 (Jag1) and is essential in the signal-emitting cells for Jag1 to activate Notch signalling. In zebrafish, loss- and gain-of-function analyses showed that Mib-Jag1-Notch signalling favours the development of non-vacuolated cells at the expense of vacuolated cells in the notochord. This leads to changes in the peri-notochordal basement membrane formation and patterning surrounding the muscle pioneer cells. These data reveal a previously unrecognized mechanism regulating the patterning and structural roles of the notochord by Mib-Jag1-Notch signalling-mediated cell-fate determination.