ArdA proteins from different mobile genetic elements can bind to the EcoKI Type I DNA methyltransferase of E. coli K12.
ArdA proteins from different mobile genetic elements can bind to the EcoKI Type I DNA methyltransferase of E. coli K12.
复制标题
DOI：
10.1016/j.bbapap.2013.12.008
复制
发表时间：
2014-03
期刊：
影响因子：
--
通讯作者：
Dryden DT
中科院分区：
文献类型：
--
作者：
Chen K;Reuter M;Sanghvi B;Roberts GA;Cooper LP;Tilling M;Blakely GW;Dryden DT
抗限制和抗修饰（anti-RM）是防止外来DNA进入新细菌宿主的DNA限制-修饰（RM）系统切割的能力。抗RM的进化结果是增强了移动的遗传元件的传播。ArdA抗RM蛋白的同源物由存在于许多移动的遗传元件（例如细菌基因组内的接合质粒和转座子）中的基因编码。ArdA蛋白通过模拟I型RM酶结合的DNA结构引起抗RM。我们研究了粪肠球菌V583、金黄色葡萄球菌Mu 50和脆弱拟杆菌NCTC 9343基因组中的ArdA蛋白，并将其与接合转座子Tn 916表达的ArdA蛋白进行了比较。我们发现，尽管具有非常不同的结构稳定性和二级结构含量，它们都可以结合到EcoKI甲基转移酶，EcoKI I型RM系统的核心组成部分。这一发现表明，结构较低的ArdA蛋白在结合时完全折叠。来自不同移动的元件的ArdA抑制来自其他细菌的I型RM系统的能力表明，它们不仅有利于在密切相关的细菌之间转移，而且有利于在更远相关的细菌物种之间转移。不同的ArdA蛋白都靶向EcoKI I型DNA修饰酶。ArdA蛋白具有可变的二级结构含量。ArdA与EcoKI的结合同样良好，尽管稳定性存在差异。
Anti-restriction and anti-modification (anti-RM) is the ability to prevent cleavage by DNA restriction–modification (RM) systems of foreign DNA entering a new bacterial host. The evolutionary consequence of anti-RM is the enhanced dissemination of mobile genetic elements. Homologues of ArdA anti-RM proteins are encoded by genes present in many mobile genetic elements such as conjugative plasmids and transposons within bacterial genomes. The ArdA proteins cause anti-RM by mimicking the DNA structure bound by Type I RM enzymes. We have investigated ArdA proteins from the genomes of Enterococcus faecalis V583, Staphylococcus aureus Mu50 and Bacteroides fragilis NCTC 9343, and compared them to the ArdA protein expressed by the conjugative transposon Tn916. We find that despite having very different structural stability and secondary structure content, they can all bind to the EcoKI methyltransferase, a core component of the EcoKI Type I RM system. This finding indicates that the less structured ArdA proteins become fully folded upon binding. The ability of ArdA from diverse mobile elements to inhibit Type I RM systems from other bacteria suggests that they are an advantage for transfer not only between closely-related bacteria but also between more distantly related bacterial species. Diverse ArdA proteins all target the EcoKI Type I DNA modification enzyme. ArdA proteins have variable secondary structure content. ArdA all bind equally well to EcoKI despite stability variations.
登录
查看更多内容
影响因子：
14.9
作者：
Atanasiu, C;Su, TJ;Dryden, DTF
通讯作者：
Dryden, DTF
影响因子：
10.5
作者：
Kennaway, Christopher K.;Taylor, James E.;Dryden, David T. F.
通讯作者：
Dryden, David T. F.
影响因子：
5.6
作者：
Nekrasov, Serge V.;Agafonova, Olga V.;Belogurov, Anatol A.
通讯作者：
Belogurov, Anatol A.
影响因子：
14.9
作者：
McMahon SA;Roberts GA;Johnson KA;Cooper LP;Liu H;White JH;Carter LG;Sanghvi B;Oke M;Walkinshaw MD;Blakely GW;Naismith JH;Dryden DT
通讯作者：
Dryden DT
影响因子：
3.9
作者：
Dryden, DTF;Tock, MR
通讯作者：
Tock, MR