The affinity technology in downstream processing.
The affinity technology in downstream processing.
复制标题
下游加工中的亲和技术。
DOI：
10.1016/0168-1656(94)90047-7
复制
发表时间：
1994
影响因子：
4.1
通讯作者：
Y. Clonis
中科院分区：
文献类型：
--
作者：
N. Labrou;Y. Clonis
由于生物化工产品的质量要求非常严格，因此，高分离纯化技术对下游加工至关重要。基于亲和性的净化技术被认为是最好的可用技术，每种技术在净化能力、经济性、处理速度和容量方面都有所不同。最广泛使用的亲和技术是经典的亲和色谱法，然而，也开发了其他基于色谱法的方法，例如灌注亲和色谱法、hyperdiffusion™亲和色谱法、高效亲和色谱法、离心亲和色谱法、亲和排斥色谱法、异双功能配体亲和色谱法和“亲和尾”的各种色谱应用。另一方面，非色谱亲和技术的目标是高通量，并寻求规避与大多数色谱填料所经历的扩散限制相关的问题。连续亲和循环萃取、双水相亲和分配、膜亲和过滤、亲和错流超滤、可逆可溶性亲和聚合物分离和亲和沉淀都是非色谱技术。在不同程度上使用了几种类型的亲和配体;抗体及其片段、受体及其结合物质、抗生物素蛋白/生物素系统、纺织品和仿生染料、（寡）肽、反义肽、螯合金属阳离子、凝集素和苯基硼酸盐、蛋白A和G、钙调蛋白、DNA、序列特异性DNA、（寡）核苷酸和肝素。同样，有几种支持类型的开发和使用;天然，合成无机和复合材料。
The quality criteria imposed on several biochemicals are stringent, thus, high-separation purification technology is important to downstream processing. Affinity-based purification technologies are regarded as the finest available, and each one differs in its purifying ability, economy, processing speed and capacity. The most widely used affinity technology is classical affinity chromatography, however, other chromatography-based approaches have also been developed, for example, perfusion affinity chromatography, hyperdiffusion™ affinity chromatography, high-performance affinity chromatography, centrifugal affinity chromatography, affinity repulsion chromatography, heterobi-functional ligand affinity chromatography and the various chromatographic applications of ‘affinity tails’. On the other hand, non-chromatographic affinity technologies aim at high throughput and seek to circumvent problems associated with diffusion limitations experienced with most chromatographic packings. Continuous affinity recycle extraction, aqueous two-phase affinity partitioning, membrane affinity filtration, affinity cross-flow ultrafiltration, reversible soluble affinity polymer separation and affinity precipitation are all non-chromatographic technologies. Several types of affinity ligands are used to different extents; antibodies and their fragments, receptors and their binding substances, avidin/biotin systems, textile and biomimetic dyes, (oligo)peptides, antisense peptides, chelated metal cations, lectins and phenylboronates, protein A and G, calmodulin, DNA, sequence-specific DNA, (oligo)nucleotides and heparin. Likewise, there are several support types developed and used; natural, synthetic inorganic and composite materials.
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通过添加高浓度的非特异性蛋白质来提高亲和纯化的 DNA 结合蛋白的活性。
影响因子：
2.9
作者：
Zhang,XY;Asiedu,CK;Supakar,PC;Ehrlich,M
通讯作者：
Ehrlich,M
DOI：
10.1016/s0021-9258(18)81778-1
发表时间：
1989-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Lohman;Kinlin Chaos;Michael Green;S. Sage;G. Runyon
通讯作者：
T. Lohman;Kinlin Chaos;Michael Green;S. Sage;G. Runyon
结合凝集素亲和和金属相互作用色谱，通过高效液相色谱分离血型糖蛋白。
DOI：
10.1016/s0021-9673(00)90549-1
发表时间：
1988
期刊：
Journal of chromatography
影响因子：
--
作者：
Corradini,D;elRassi,Z;Horváth,C;Guerra,G;Horne,W
通讯作者：
Horne,W
DOI：
10.1016/0021-9673(91)85017-a
发表时间：
1991-12-27
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
GOSS, TA;BARD, M;JARRETT, HW
通讯作者：
JARRETT, HW